Identification of cytotoxic metabolites from Mahonia aquifolium using 1H NMR-based metabolomics approach
- 1. Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia
- 2. Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, Serbia
- 3. Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia
- 4. Institute for Medicinal Plant Research "Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia
Description
Herein, we propose a 1H NMR-based metabolomics method to reveal cytotoxic metabolites from Mahonia aquifolium stem-bark. Primary and secondary metabolites in the Mahonia aquifolium extracts were identified by thorough analysis of 1H and 2D NMR spectra, without prior isolation. An OPLS multivariate analysis method was used to correlate the chemical composition of the plant extracts with the results of cytotoxic activity against Human cervical adenocarcinoma cell line. Protoberberine alkaloids berberinе and palmatine, along with bisbenzylisoquinoline alkaloid berbamine were identified as the most influential in the OPLS model, with the highest cytotoxic activity.
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- 0731-7085 (ISSN)
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- Ministry of Education, Science and Technological Development
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination 172053
- Ministry of Education, Science and Technological Development
- Biological response modifiers in physiological and pathological conditions 175011