Info: Zenodo’s user support line is staffed on regular business days between Dec 23 and Jan 5. Response times may be slightly longer than normal.

Published August 7, 2020 | Version v1
Journal article Open

Optical topography of rough surfaces using vortex localization of fluorescent markers

  • 1. Department of Optics, Palacký University, 17. listopadu 1192/12, 771 46 Olomouc, Czech Republic
  • 2. Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, 612 00 Brno, Czech Republic

Description

Measuring rough surfaces is challenging because the proven topographic methods are impaired by the adverse effects of diffuse light. In our method, the measured surface is marked by fluorescent nanobeads allowing a complete suppression of diffuse light by bandpass filtering. Light emitted by each fluorescent bead is shaped to a double-helix point spread function used for three-dimensional bead localization on the surface. This non-interferometric measurement of rough surface topography is implemented in a vibration resistant setup. The comparison of our method with vertical scanning interferometry shows that a commercial profiler is surpassed when ground glass surfaces with steep slopes are measured.

Notes

Funding: Grantová Agentura Ceské Republiky (GA18- 01396S); Central European Institute of Technology (LQ1601); H2020 Spreading Excellence and Widening Participation (No. 810626); Univerzita Palackého v Olomouci (IGA PrF 2020 004); Czechoslovak Microscopy Society

Files

OpticsLett Schovánek.pdf

Files (1.3 MB)

Name Size Download all
md5:69692d0b9dc6cb02f438d7f3747f92d9
1.3 MB Preview Download