Spectral data and R modeling code from: Polarized light sensitivity in Pieris rapae is dependent on both color and intensity

File Descriptions

This read me file includes the following information detailing the contents of the included files and the methods used in their collection.

read me.txt

Transmission of the walls of the experimental arena

Transmission of the experimental arena walls, expressed as a percentage. This measurement was taken with a HR-4000 spectrophotometer (Ocean Optics Inc., Dunedin, FL, USA).

aquarium glass.csv

Transmission of the λ/4 retarder film

Transmission of the λ/4 retarder film (#88-253, Edmund Optics, Barrington, NJ), expressed as a percentage. This measurements was taken with a HR-4000 spectrophotometer (Ocean Optics Inc., Dunedin, FL, USA).

qwp edmund optics 88-253.csv

Reflectance of kraft paper background

Reflection of the kraft paper background expressed as a percentage. This measurement was taken with a JAZ spectrometer (Ocean Optics Inc., Dunedin, FL, USA) calibrated with a 99% Spectralon reflectance standard (SRS-99-010, Labsphere, NH, USA).

kraft paper.csv

Irradiance of the fluorescent lamps

Irradiance (photons/cm2/s/nm) of the fluorescent lamps (F32T8/SPX50/ECO GE, Boston, MA) used to light the experimental arena. This measurement was taken with a HR-4000 spectrophotometer (Ocean Optics Inc., Dunedin, FL, USA).

fluorescent tubes.csv

Irradiance of the LCD monitors

Irradiance (photons/cm2/s/nm) of the LCD monitors (1707FPt, Dell Inc., Round Rock, TX) used to display experimental stimuli while displaying white pixels (RGB: 255,255,255). This measurement was taken with a HR-4000 spectrophotometer (Ocean Optics Inc., Dunedin, FL, USA).

monitors RGB 255 spectra.csv

λ/4 retarder film measurements, raw data

The R code below also depends on these measurements of the λ/4 retarder film (#88-253, Edmund Optics, Barrington, NJ) from Blake et al. (2019, https://doi.org/10.5061/dryad.xgxd254bs) but not included here. The file consists of 136 transmission measurements taken through a λ/4 retarder film sandwiched between two polarizers. The measurements were taken at different rotations of the λ/4 retarder film (97°, 93°, 91°, 90°, 89°, 85°, 81°, 77°, 73°, 69°, 65°, 61°, 57°, 53°, 49°, 46°, 45°, 44°, 43°, 41°, 39°, 35°, 31°, 27°, 23°, 19°, 15°, 11°, 7°, 3°, 1°, 0°, -1°, -5°), and different angles between the axes of the two polarizers (0°, 45°, 90°, 135°).

QWP_Retarder_Film_Measurement_data.csv

Spectral sensitivity of female Pieris rapae photoreceptors

Spectral sensitivity (0-1) of the various photoreceptors classes in Pieris rapae females from Blake et al. (2019, https://doi.org/10.1007/s00359-019-01330-8). Ultraviolet (UV), Violet (V), Blue (B), Green (G), type I ommatidia Red (Ri), type III ommatidia Red (Riii)

female spectral sensitivity.csv

Modeled spectral sensitivity of female type II Pieris rapae photoreceptors

Modeled spectral sensitivity (0-1) of type II female Pieris rapae photoreceptors from Stavenga and Arikawa (2011, https://doi.org/10.1007/s00359-010-0622-5). Only the R5-8 sensitivity is used for the spectral sensitivity of the type II Red photoreceptors (Rii).

type II female model spectra.csv

Figure 1b, R code

R code to generate spectral sensitivity graph from data in "female spectral sensitivity.csv" and "type II female model spectra.csv".

Fig. 1.R

Modeling photoreceptor quantum catches, R code

R code to model the photoreceptor quantum catch in Pieris rapae females using all of the data files mentioned above. This data is used to generate Figs. 5, S2 and S3.

Fig. 5, S2, S3.R

Figure S1, R code

R code to generate spectroscopy graphs from "aquarium glass.csv", "QWP Edmund Optics 88-253.csv", "kraft paper.csv", "fluorescent tubes.csv", and"monitors RGB 255 spectra.csv".

Fig. S1.R

Funding provided by: National Science and Engineering Research Council of Canada
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100000038
Award Number: IRC

Funding provided by: Scotts Canada Ltd.*
Crossref Funder Registry ID:
Award Number: IRC

Funding provided by: Scotts Canada Ltd.
Crossref Funder Registry ID:
Award Number: IRC