Isolation and Identification of Dye Degrading Bacteria from Wastewater Samples Across Delhi NCR

Industrial effluents containing unreacted azo dyes contaminates the water heavily and is one of the major sources of environmental pollution. Hence bioremediation by removal of dye effluents from water is essential. The aim of this research is to isolate bacterial strains that can efficiently degrade azo dyes and identify the strains using Advanced Bacterial Identification Software (ABIS). Wastewater Samples were collected from five different zones rich with Dye Effluents across Delhi NCR region. 26 bacterial strains were isolated out of which six strains were positive for dye decolorization activity. Biochemical Tests were done for identifying the characteristics of the positive strains. Quantitative analysis was also done for those strains using three dyes (Yellow, Blue and Green) to find out the rate of dye decolorization of each strain. Isolates Culture-13 (C-13) and Culture-23 (C-23), identified as Bacillus spp. and Staphylococcus spp. respectively, were among the best dye degraders. C-23 was efficient enough to degrade Green dye up to 97% in a week.