Single-cell metabolic profiling (scMEP) of human cytotoxic T cells

Datasets for Hartmann FJ et al. (2020) Single-cell metabolic profiling of human cytotoxic T cells. Nature Biotechnology

Contains single-cell mass cytometry (CyTOF) datasets for metabolic analysis of human whole blood populations, in vitro T cell activation and analysis of metabolic states in human tissues as well as MIBI-TOF multiplexed images and segmented single-cell data of colorectal carcinoma and healthy colon.

• All CyTOF datasets have been manually gated on single, live cells to enable direct import into data analysis software (e.g. R environment)
• MIBI-TOF images have undergone noise removal as described in Keren et al. (2018) Cell
• Segmentation masks for MIBI-TOF data contain large non-cellular regions that need to be removed during downstream processing
• MIBI-TOF derived single-cell data is cell size normalized, arcsinh transformed and percentile normalized and contains manually annotated FlowSOM clustering results