Supplementary Files_Fernandes_ColonCancer_Revised

Figure S1: PCA scores plot for plasma samples. Colon cancer patients and control volunteers in the positive (A) and negative (B) ion modes.

Figure S2: PLS-DA scores plots in the negative ion mode of CTR volunteers (green) and CC patients (red). A: 48.2 and 15.7 % represent the scores of components 1 and 2, respectively. B: Performance measures of the PLS-DA model (prediction accuracy, R2, and Q2), *best value of Q2 (0.97). C: Permutation test statistics at 1000 permutations (p < 0.001).

Figure S3: Hierarchical clustering of samples shown as a heatmap. CC patients and CTR volunteers in green and red bars respectively, as showed in the top. In the bottom: samples from male subjects indicated by blue bars and from females by yellow bars. a: Cluster with mainly male controls in the left. b: Cluster with mainly female controls in the right.

Figure S4. PCA scores plot for plasma samples. Male and female CC patients (A) and CTR volunteers (B) in the negative mode.

Figure S5: Volcano plots in the negative mode (A) significant features (pink dots) (B) potential biomarkers identified in this study indicated by colored circles (C) potential biomarkers and their putative identifications (colored labeled circles) and non-significant features as small dots.

Figure S6:Figure S6: Volcano plots in the negative mode (A) significant features (pink dots) (B) potential biomarkers identified in this study indicated by colored circles (C) potential biomarkers and their putative identifications (colored labeled circles) and non-significant features as small dots.

Spreadsheet_1: USF_Colon_LCMS_negative_processed.

Spreadsheet_2: USF_Colon_LCMS_positive_processed

Table S1: Statistical data of differential features between CC patients and CTR volunteers. 
Table S2: Pathway Analysis results.