Published June 4, 2020 | Version V1.0
Technical note Open

Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology

  • 1. MNI, McGill University

Description

This protocol describes how to generate a gene knockout cell line through a combination of techniques that includes:

  • CRISPR-Cas9 editing;
  • Droplet digital PCR (ddPCR) technology;
  • Single cell sorting.

Files

EDDU-013-01_CRISPR editing of non-iPSCs through ddPCR_v1.0.pdf

Files (2.3 MB)