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Published November 23, 2017 | Version v6
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Stem trichome mRNA-Seq from lycopersicum x habrochaites crosses (Parents and F1)

  • 1. University of Amsterdam

Description

Stem trichome mRNA-Seq datasets from different crossing materials.

Genetic material

Obtained from ENZA Zaden. The pollen from S. habrochaites LA1777 or PI127826 was placed on the pistil of S. lycopersicum to obtain interspecific crosses. 

The pollen of S. habrochaites LA1777 was placed on the pistil of PI127826 to obtain S. habrochaites F1.

Sample collection

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Total RNA isolation

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Sequencing

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Sample table

| sample            | species                           | cross  | forward_read                                             | reverse_read                                            | comment     |
|-------------------|-----------------------------------|--------|----------------------------------------------------------|---------------------------------------------------------|-------------|
| 14865_PI127826_F1 | S. lycopersicum x S. habrochaites | F1     | 14865_PI127826_F1_AGCGATAG-AGGCGAAG_L007_R1_001.fastq.gz | 14865_PI127826_F1_AGCGATAG-AGGCGAAG_L007_R2.fastq.gz    |             |
| LA1777_4          | S. habrochaites                   | Parent | LA1777_4_TCTCGCGC-CAGGACGT_L005_R1_001.fastq.gz          | LA1777_4_TCTCGCGC-CAGGACGT_L005_R2_001.fastq.gz         |             |
| LA1777_F1_3       | S. lycopersicum x S. habrochaites | F1     | LA1777_F1_3_TCTCGCGC-TAATCTTA_L007_R1_001.fastq.gz       | LA1777_F1_3_TCTCGCGC-TAATCTTA_L007_R2_001.fastq.gz      |             |
| LA1777_P1_Cv_4    | S. lycopersicum                   | Parent | LA1777_P1_Cv_4_TCTCGCGC-AGGCGAAG_L007_R1_001.fastq.gz    | LA1777_P1_Cv_4_TCTCGCGC-AGGCGAAG_L007_R2_001.fastq.gz   |             |
| PI26_P1_cv_i      | S. lycopersicum                   | Parent | PI26_P1_cv_i_AGCGATAG-CCTATCCT_L006_R1_001.fastq.gz      | PI26_P1_cv_i_AGCGATAG-CCTATCCT_L006_R2_001.fastq.gz     |             |
| PI26_P2_w_4       | S. habrochaites f. hirsutum       | Parent | PI26_P2_w_4_AGCGATAG-TATAGCCT_L005_R1_001.fastq.gz       | PI26_P2_w_4_AGCGATAG-TATAGCCT_L005_R2_001.fastq.gz      |             |
| PI26xLA1777_F1_J  | S. habrochaites                   | F1     | PI26xLA1777_F1_J_TCTCGCGC-GTACTGAC_L005_R1_001.fastq.gz  | PI26xLA1777_F1_J_TCTCGCGC-GTACTGAC_L005_R2_001.fastq.gz |             

 

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Additional details

Funding

Dutch Research Council
Defence in the wild; from trichome transcriptomes and metabolomes to breeding tools for defence markers in tomato 2300178970