Published July 1, 2019 | Version preprint
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Encapsulation, Visualization and Expression of Genes with Biomimetically Mineralized Zeolitic Imidazolate Framework‐8 (ZIF‐8)

Creators

  • 1. Ian Potter NanoBiosensing Facility NanoBiotechnology Research Laboratory (NBRL), School of Science, RMIT University, Australia
  • 2. ALBA Synchrotron Light Source MISTRAL Beamline – Experiments division, Spain
  • 3. School of Chemical Engineering and Graduate School of Biomedical Engineering, University of New South Wales Australia
  • 4. ARC Centre of Excellence for Nanoscale BioPhotonics, School of Science, RMIT University, Australia
  • 5. Centre for Molecular and Nanoscale Physics, School of Science, RMIT University, Australia
  • 6. Institute of Physical and Theoretical Chemistry, Graz University of Technology, Austria
  • 7. Institute of Inorganic Chemistry, Graz University of Technology, Austria
  • 8. Department of Chemistry and the Centre for Advanced Nanomaterials, The University of Adelaide, Australia
  • 9. CSIRO Manufacturing Clayton, Australia

Description

Recent work in biomolecule-metal–organic framework (MOF) composites have proven to be an effective strategy for the protection of proteins. However, for other biomacromolecules such as nucleic acids, the encapsulation into MOFs and the related characterizations are at is infancy.  We herein report encapsulation of complete gene-set in zeolitic imidazolate framework-8 (ZIF-8) MOFs and intracellular expression of the gene delivered by the MOF composites. Using a GFP plasmid (plGFP) as a proof-of-concept genetic macromolecule, we show successful transfection of mammalian cells with plGFP for up to 4 days, demonstrating the feasibility of DNA@MOF biocomposites as intracellular gene delivery vehicles which occurs over relatively prolonged time points where the cargo nucleic acid is released gradually in order to maintain sustained expression.

Notes

This is the pre-peer reviewed version of the following article: Encapsulation, Visualization and Expression of Genes with Biomimetically Mineralized Zeolitic Imidazolate Framework-8 (ZIF-8), which has been published in final form at http://dx.doi.org/10.1002/smll.201902268. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

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