A modular open source technology for automated in vitro workflows

Automation liberates scientific staff from repetitive tasks, decreases the probability of human error and consequently enhances the reproducibility of lab experiments. However, the use of laboratory automation in academic laboratories is limited due to high acquisition costs and the inability to customize off-the-shelf hardware. To address these challenges, we present an Open Source Hardware (OSH) concept for an assembly line-inspired workstation consisting of ready-to-use and customizable modules. In contrast to current standalone solutions, this concept enables the combination of single hardware modules – each with a specific set of functionalities – to a modular workstation to provide a fully automated setup. The base setup consists of a pipetting and transport module and is designed to execute basic protocol steps for in vitro research applications, including pipetting operations for non-viscous and viscous liquids and transportation of cell culture vessels between the modules. We demonstrate the successful application of this concept within a case study by the development of a storage module to facilitate high-throughput studies and a crosslinker module to initiate polymerization of hydrogel solutions. We present a Systems Engineering framework for customized module development, guidance for the design and assembly of the presented modules, and operational instructions on the usage of the workstation. By combining capabilities from various open source instrumentations into a modular technology platform, this OSH approach will facilitate efficient and reliable experimentation for in vitro research. Ultimately, this OSH concept will allow academic groups to improve replicability and reproducibility in cell culture process operations towards more economical and innovative research in the future.