Evaluation of antioxidant activity and brine shrimp lethality bioassay of Randia dumetorum stem extract

This research study accomplished to explore the antioxidant activity as well as cytotoxicity assessment like brine shrimp lethality bioassay of distinct fractions of Randia dumetorum stem extract. To fractionate through soxhletion using sequential extraction techniques where the plant stem used as a powdered form with some specific solvents treatment. Here, we used to evaluate antioxidant activity, total antioxidant capacity determination, reducing power assay, ferric ions reduction using a simple method named orthophenanthroline color method, determination of total content of phenol and total flavonoids contents by aluminium tricholoride method. In these studied we incorporate Ascorbic and Gallic acid as an antioxidant compound. It observed that, the content of proximate analysis moisture is 10.3%, total ash value is 4.76%, acid insoluble ash is 4.30% and water soluble ash value is 3.21%. For phytochemical screening, different extract of those solvents were utilized that disclosed the existence of alkaloids, reducing sugar, flavonoids, saponin, phenols, Tannic acid, amino acid and protein. In the case of brine shrimp lethality bioassay, methanol extract of stem effect to brine shrimp nauplii and exhibiting highest toxicity having LC50 value 1.32 μg/ml as compared to standard dimethyl sulfoxide (LC50 1.31 µg/ml). These evaluations suggest that Randia dumetorum stems indicated a better source of antioxidants and hold important cytotoxic effect.