Published October 31, 2019
| Version v1
Journal article
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Innovative and Frugal Protocols for RNA Related Experiments
- 1. University of Agricultural Sciences, GKVK, Bengaluru-560065, INDIA
- 2. University of Horticultural Sciences, Bagalkot, GKVK post, Bengaluru-560065, INDIA
- 3. IFOM-inStem Joint Research Laboratory, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore, Karnataka, India
Description
Rice is staple crop and is being cultivated far and wide around the globe. Waterlogged habit of cultivation has made rice extremely sensitive to drought. Understanding expression profiles will open new avenues of research for drought tolerance in rice. Present study aims at utilizing the most innovative and frugal ways for highly sensitive molecular biology protocols. These protocols will help researchers to undertake RNA work in any lab with basic amenities. A details methodologies with critical tips is compiled and presented here for RNA extraction, RNA detection and finally expression profiles of two genotypes. This proof of concept for novel methods is a complete guide for beginner in RNA work with minute detailing of each step
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