A Compendium to the Phosphoregulation of Mitosis

The regulation of mitosis is a complex, highly ordered process involving thousands of phosphorylation and dephosphorylation events. Many details about the process are still unknown, however current estimates from large-scale, deep phospho-proteomics analysis suggest that the majority of the proteome gets fully-phosphorylated during mitosis (Olsen et al., 2010). To help in understanding how mitosis is regulated, we selected 88 phospho-events believed to play key roles, and used them to create a tailored visualization (Burgess et al., 2017) based on the ‘Minardo’ layout, in which time is represented as flowing around a schematic ‘circtanglar’ cell (Ma et al., 2013). The visualization provides a quick yet comprehensive overview of the events regulating entry, progression and exit from mitosis (Burgess et al., 2017). Each phospho-event is visually linked to its corresponding kinase or phosphatase, adding an additional layer of information. An interactive version is also available (http://www.cell.com/cell/enhanced/odonoghue2);here, users can click on each phosphosite to open up a popup that contains a brief description of the event, as well as links to supporting literature in PubMed, related 3D structures in Aquaria (O’Donoghue et al., 2015),  and protein sequence information in UniProt (The UniProt Consortium, 2019). Below, in this publication, we have compiled all the text from the 88 popups in the online interactive visualization, with the aim of making this information more easily accessible, as well as acknowledging the contributions of the >200 scientific publications that we used in assembling the visualization. This provides a comprehensive compendium of the major phospho-events currently believed to be essential for mitosis.