Published July 3, 2019 | Version v0.2
Dataset Open

Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing

Description

Supplementary data that are needed to rerun the reproducible notebooks from the first steps using Alevin output and configuration files.

Intermediate R data object that can be used to rerun the reproducible notebooks after the filtering steps.

Validation data for inferring the sample index hopping rate. The hiseq4000_joined_datatable_plexed_nonplexed.zip file contains read counts for four samples (two non-multiplexed and two multiplexed)  joined by  a cell-barcode, UMI, and gene-ID (CUG) key combination. The hiseq4000_inner_joined_with_labels.zip file contains only those CUGs that are observed in both the non-multiplexed and multiplexed samples.

Files

alevin_files.zip

Files (1.2 GB)

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md5:975a30456e26aae4bb3888ec75ca7a3c
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Additional details

Related works

Is referenced by
10.1101/617225 (DOI)
Is supplemented by
https://csglab.github.io/phantom_purge/index.html (URL)