Published January 1, 2015 | Version v1
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Histological properties of intramuscular connective tissues in native chickens and their relationship with meat tenderness

  • 1. Department of Basic Sciences, College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord – Iran

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The most common connective tissue research in meat science has been conducted on the properties of intramuscular connective tissue of meat. The purpose of this study was to investigate histological properties of intramuscular connective tissues of left and right Quadriceps femoris muscle (as red meat) and Pectoralis superficialis muscles (as white meat) in the native chickens and the influence of sex on these properties. A total of 40 adult healthy native chickens (56 days) of both sexes (20 female and 20 male) were used. After fixation in 10% buffered formalin solution, sections were prepared, using routine histological techniques. Tissue samples were stained by hematoxylin eosin and a variety of special techniques for determination of types of connective tissue fibers. The conventional histological study revealed that except the endomysium which was similar in both muscles, the other intramuscular connective tissues layers varied between leg and breast muscles and affected by sex. All the connective tissue fibers observed in the all intramuscular connective tissues of both red and white meats. Tenderness in the leg muscle was higher than the breast muscle in native chickens, as well as in males than females. Elastic fibers showed no histological difference not only among intramuscular connective tissues in both red and white muscles meats but also between males and females.

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References

  • ALBERTS, B., JOHNSON, A., LEWIS, J., RAFF, M., ROBERTS, K., and WALTER, P. 2002. Molecular biology of the cell. Fourth ed. Garland Science, New York (NY).
  • BAILEY, A. J. and LIGHT, N.D. 1989. Connective tissue in meat and meat products. Elsevier Applied Science, London.
  • Coro, F. A., Youssef, E. Y. AND Shimokomaki, M. 2000. Age related changes in breast poultry meat collagen crosslink, hydroxylysylpyridinium. Proceedings of 46th International Congress of Meat Science and Technology. (Pp. 432-433), 27 August-1 September, Buenos Aires, Argentina.
  • CORREA, J. A., FAUCITANO, L., LAFOREST, J. P., RIVEST, J., MARCOUX, M. AND GARIEPY, C. 2006. Effects of slaughter weight on carcass composition and meat quality in pigs of two different growth rates. Meat Sci., 72(1): 91-99.
  • DELLMANN, H.D. 1993. Textbook of veterinary histology. 4th ed., Lea and Febiger, Philadelphia, Pp. 30-31, 73.
  • FERNANDEZ, X., SANTE, V., BAEZA, E., LEBIHAN-DUVAL, E., BERRI,C., REMIGNON,H.,BABILE, R., LE POTTIER, G., MILLET, N., BERGE, P. AND ASTRUC, T. 2001. Post mortem muscle metabolism and meat quality in three genetic types of turkey. Br. Poul. Sci., 42(4): 462-469.
  • IWAMOTO, H., TABATA, S., SAKAKIBARA, K., NISHIMURA, S., GOTOH, T. AND KOGA, Y. 2001. Scanning electron microscopic observation of the architecture of collagen fibres in chicken M. iliotibialis lateralis. Br. Poul. Sci., 42(3): 321-326.
  • JARVINEN, T. A. H., JOZSA, L., KANNUS, P., JARVINEN, T. L. N. AND JARVINEN, M. 2002. Organization and distribution of intramuscular connective tissue in normal and immobilized skeletal muscles. J Muscle Res Cell Motil., 23(3): 245-254.
  • KERR, C. L., HAMMERSTEDT, R. H. AND BARBATO, G. F. 2001. Effects of selection for exponential growth rate at different ages on reproduction in chickens. Avian Poult Biol Rev., 12(3): 127-136.
  • KIERNAN, J.A. 2008. Histological and Histochemical Methods: Theory and Practice. 4th ed. Bloxham, UK, Scion.
  • LAWRIE, R. A. 1998. Lawrie's Meat Science. Woodhead Publishing Limited. Cambridge, England.
  • Li, C. B., Zhou, G. H. AND Xu, X. L. 2007. Comparisons of meat quality characteristics and intramuscular connective tissue between beef longissimus dorsi and semitendinosus muscles from Chinese yellow bulls. J Muscle Foods, 18(2): 143-161.
  • LIU, A., NISHIMURA, T. AND TAKAHASHI, K. 1996. Relationship between structural properties of intramuscular connective tissue and toughness of various chicken skeletal muscles. Meat Sci., 43(1): 43-49.
  • MAHON, M. 1999. Muscle abnormalities: morphological aspects. In: Poultry Meat Science. eds R.I. Richardson and G.C. Mead. Vol 25 Poultry Science Symposium Series CABI Publishing Abingdon, Oxford shire, UK, Pp. 19-64.
  • MINVIELLE, B., LE STRAT, P., LEBRET, B., HOUIX, Y., BOULARD, J. AND CLOCHEFERT, N. 2001. Viandes destructurees. Situation dans cinq abattoirs de l'Ouest de la France: facteurs de risque et proposition d'un modele. Caracterisation colorimetrique, biochimique et histologique. Journées de la Recherche Porcine, 33: 95-101.
  • MOBINI, B. 2013. Histological differences in intramuscular connective tissues composition between dark and light colored muscles in broiler chickens. Global Veterinaria, 10(3): 360-364.
  • NAKAMURA, Y. N., IWAMOTO, H., SHIBA, N., MIYACHI, H., TABATA, S. AND NISHIMURA, S. 2004a. Developmental states of the collagen content, distribution and architecture in the pectoralis, iliotibialis lateralis and puboischiofemoralis muscles of male Red Cornish x New Hampshire and normal broilers. Br. Poul. Sci., 45(1): 31-40.
  • NAKAMURA, Y. N., IWAMOTO, H., SHIBA, N., MIYACHI, H., TABATA, S. AND NISHIMURA, S. 2004b. Growth changes of the collagen content and architecture in the pectoralis and iliotibialis lateralis muscles of cockerels. Br. Poul. Sci., 45(6): 753-761.
  • NAKAMURA, Y. N., IWAMOTO, H., TABATA, S., ONO, Y., SHIBA, N. AND NISHIMURA, S. 2003. Comparison of collagen content, distribution and architecture among the Pectoralis, Iliotibialis lateralis and Puboischiofemoralis muscles with different myofiber composition in Silky cocks. Anim. Sci. J., 74(2): 119-128.
  • ONO, Y., IWAMOTO, H. AND TAKAHARA, H. 1993. The relationship between muscle growth and the growth of different fiber types in the chicken. Poultry Sci., 72: 568-576.
  • OSHIMA, I., IWAMOTO, H., NAKAMURA, Y. N., TAKAYAMA, K., ONO, Y., MURAKAMI, T., SHIBA, N., TABATA, S. AND NISHIMURA, S. 2009. Comparative study of the histochemical properties, collagen content and architecture of the skeletal muscles of wild boar crossbred pigs and commercial hybrid pigs. Meat Sci., 81(2): 382-390.
  • PAPINAHO, P., RUUSUNEN, M., SUURONEN, T. AND FLETCHER, D. 1996. Relationship between muscle biochemical and meat quality properties of early-deboned broiler breasts. J Appl Poultry Res., 5: 126-133.
  • RAES, K., BALCAEN, A., DIRINCK, P., DE WINNE, A., CLAEYS, E., DEMEYER, D. AND DE SMET, S. 2003. Meat quality, fatty acid composition and flavour analysis in Belgian retail beef. Meat Sci., 65(4): 1237-1246.
  • ROWE, R. W. D. 1981. Morphology of perimysial and endomysial connective tissue in skeletal muscle. Tissue Cell. 13(4): 681-690.
  • ROY, B. C., OSHIMA, I., MIYACHI, H., SHIBA, N., NISHIMURA, S., TABATA, S. AND IWAMOTO, H. 2007. Histochemical properties and collagen architecture of M. Iliotibialis lateralis and M. Puboischiofemoralis in male broilers with different growth rates induced by feeding at different planes of nutrition. Br. Poul. Sci., 48(3): 312-322.
  • SAKAKIBARA, K., TABATA, S., SHIBA, N., GOTOH, T., NISHIMURA, S. AND IWAMOTO, H. 2000. Myofiber composition and total collagen content in M. Iliotibialis lateralis and M. Pectoralis of Silkie and White Leghorn chickens. Br. Poul. Sci., 41(5): 570- 574.
  • THERKILDSEN, M., RIIS, B., KARLSSON, A., KRISTENSEN, L., ERTBJERG, P., PURSLOW, P. P., DALL AASLYNG, M. AND OKSBJERG, N. 2002. Compensatory growth response in pigs, muscle protein turn-over and meat texture: effects of restriction/realimentation period. Anim Sci., 75(3): 367-377.
  • TOTLAND, G. K., KRYVI, H. AND SLINDE, E. 1988. Composition of muscle fiber types and connective tissue in bovine M. Semitendinosus and its relation to tenderness. Meat Sci., 23(4): 303-315.
  • VOUTILA, L. 2009. Properties of intramuscular connective tissue in pork and poultry with reference to weakening of structure. Academic dissertation, Faculty of Agriculture and Forestry of the University of Helsinki, 1-94.
  • ZIMMERMANN, S. D., MCCORMICK, R. M. AND VADLAMUDI, K. 1993. Age and training alter collagen characteristics in fast- and slow-twitch rat limb muscle. J Appl Physiol., 75(4): 1670-1674.