INTRACELLULAR ANTIOXIDANT MECHANISM OF RICE PROTEIN HYDROLYSATES TREATED WITH HIGH ENERGY ELECTRON BEAM
- 1. aKey Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education State Key Laboratory of Food Science and Technolog, Jiangnan University, Wuxi, 214122, China bNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China cJiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, LihuRoad1800, Wuxi, 214122, China
Description
In this paper, intracellular antioxidant mechanism of rice protein hydrolysates (RPHs) treated with high energy electron beam irradiation (EBI) was discussed using CAA assay and H2O2-induced oxidative damage modelin HepG-2 cell. Changes of RPHs structures were measured by FTIR and CD spectra. Molecular weight distribution was detected by gel permeation chromatography. After EBI treatment, CAA value of RPHs increased from 7.58±0.19 μM QE/g to 11.46±0.16 μM QE/g, indicating that the cellular antioxidant activity of RPHswas improved by EBI. Furthermore, the EB Itreated RPHs has better protective effect on oxidative stressed cells, with cell viability increased to 88.02± 1.18%, while the level of ROS, MDA and LDH decreased by2.05-fold, 49.03% and 2.16-fold, respectively, compared with H2O2 damaged group. RPHs pretreated with EBI exerted its antioxidant effect by activating intracellular antioxidant enzymes: the SOD, CAT and GSH-Px activity of cells incubated with EBI treated RPHs were significantly higher than that of the damaged group, which was increased by 100.57 U/mg pro, 16.78 U/mg pro and 21.28 U/mg pro, respectively.The basic structure of RPHs was not changed after EBI treatment, while the secondary structure change and molecular weight reduction induced by EBI result in the antioxidant activity improvement in HepG-2cells.
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