Published March 17, 2019 | Version v1
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IN VITRO CELL GROWTH-INHIBITING AND IMMUNE MODULATING EFFECTS OF HEAT-SENSITIVE BIOACTIVE SUBSTANCES ISOLATED FROM PARASITE AND INFECTED HOST TISSUES AFTER EXPERIMENTAL TRICHINELLOSIS

Authors/Creators

  • 1. Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria

Contributors

  • 1. University of Forestry, Bulgaria.

Description

Antiproliferative effects of biochemically isolated from Trichinella spiralis muscle larvae, livers and spleens from healthy and T. spiralis infected rats heat-sensitive bioactive substances (HSBSes) were studied in vitro on nonactivated and mitogen activated with phytohemagglutinin (PHA) lymphocytes and bone marrow precursor cells. Stronger antiproliferative effects were established for HSBSes from parasite and infected host tissues than from healthy rats. Stronger growth inhibiting and immune modulating activ-ities of HSBSes were determined on PHA activated lymphocytes. Also antiproliferative effects were established on bone marrow precursor cells. This probably was a result of changed liver metabolism and immunity in T. spiralis infected hosts.

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References

  • Aranzamendi, C., F. Fransen, M. Langelaar, F. Franssen, P. Van Der Ley, J. Van Putten, V. Rutten, E. Pinelli. (2012). Trichinella spiralis-secreted products modulate DC functionality and expand regulatory T cells in vitro. Parasite Immunology, 34, 210–223.
  • Balazh, A. & I. Blazhek. (1982). Control of cell proliferation by endogenous inhibitors. Mir Publishing House, Moscow, pp. 302, (In Russian).
  • Bliss, S. K., S. P. Bliss, D. P. Beiting, A. Alcaraz, J. A. Appleton. (2007). IL-10 regulates movement of intestinally derived CD4+ T cells to the liver. The Journal of Immunology, 178, 7974–7983.
  • Duan, L. X., X. M. Guan, C. S. Zhang, P. Ru, J. Ni, Y. M. Su, L. F. Chen, X. C. Zhang. (2011). Research progress on anti-tumor mechanism of Trichinella spiralis. Chinese Journal of Parasitology and Parasitic Diseases, 29, 2, 142–146. (In Chinese).
  • Gong, P., J. Zhang, L. Cao, Z. Nan, J. Li, Yang J, H. Fang, H. Jiao, T. Jiang, L. Su, X. Zhang. (2011). Identification and characterization of myeloma-associated antigens in Trichinella spiralis. Exp. Parasitol., 127, 4, 784–788.
  • Gruden-Movsesijan, A. & L. Sofronic-Milosavjevic. (2010). Experimental trichinellosis in rats – peritoneal macrophage activity. Arch. Biol. Sci., Belgrade, 62, 1, 15–22.
  • Guo, K., X. Sun, Y. Gu, Z. Wang, J. Huang, X. Zhu. (2016). Trichinella spiralis paramyosin activates mouse bone marrow-derived dendritic cells and induces regulatory T cells. Parasites & Vectors, 9, 569.
  • Ilic, N., M. Colic, A. Gruden-Movsesijan, I. Majstorovic, S.Vasilev, L. Sofronic-Milosavljevic. (2008). Characterization of rat bone marrow dendritic cells initially primed by Trichinella spiralis antigens. Parasite Immunology, 30, 9, 491–495
  • Ivanov, D., R. Milcheva, S. Petkova, M. Gabrashanska. (2014). Serum sialic acid levels in Trichinella spiralis infected rats. Comptes rendus de l'Academie bulgare des Sciences, 2014, 2, 217–222.
  • Petkova, S., E. Gabev, L. Mihov, M. Bogoeva, E. Pozio, N. Astrukova. (2005). Therapeutic activity of mebendazole on murine experimental model on the muscle stage of trichinellosis. Comptes rendus de l'Academie bulgare des Sciences, 58, 1, 97–102.
  • Sofronic-Milosavljevic, L, N. Ilic, E. Pinelli, A. Gruden-Movsesijan. (2015). Review Article. Secretory products of Trichinella spiralis muscle larvae and immunomodulation: Implication for autoimmune diseases, allergies and malignancies. Hindawi Publishing Corporation, Journal of Immunology Research, Article ID 523875, http://dx.doi.org/10.1155/2015/523875.
  • Tsocheva-Gaytandzhieva, N. T. (2005). Review Article: Fasciolosis and tumour growth. Helminthologia (Bratislava), 42, 2, 107–113.
  • Tsocheva-Gaytandzhieva, N. T. (2006). Chronic fasciolosis and endogenous inhibitors of cell proliferation. Professor Marin Drinov Academic Publishing House, Bulgarian Academy of Science, Sofia, Bulgaria, First Edition, pp. 108 (In Bulgarian).
  • Tsocheva-Gaytandzhieva, N. T. (2016). Effects of nematode infections on tumors in animals and on tumor cell cultures. Proceedings of the Seventh Workshop of Experimental Models and Methods in Biomedical Research, May 16–18, 2016, Sofia, Bulgaria.
  • Tsocheva-Gaytandzhieva, N., R. Toshkova, E. Gardeva, L. Yossifova, S. Petkova, V. Nanev. (2016). Antiproliferative activity against tumour cells of biologically active substances isolated from livers of healthy and Trichinella spiralis infected rats. Comptes rendus de l'Academie bulgare des Sciences, 69, 11, 1443–1448.
  • Verly, W. G., Y. Deschamps, J. Pushpathadam. (1971). The hepatic chalone. I. Assay method for the hormone and purification of the rabbit liver chalone. Canadian J. Biochem., 49, 1376–1383.
  • Wang, X. L., B. Q. Fu, S. J. Yang, X. P. Wu, G. Z. Cui, M. F. Liu, Y. Zhao, Y. L. Yu, X. Y. Liu, H. K. Deng, Q. J. Chen, M. Y. Liu. (2009). Trichinella spiralis – a potential anti-tumor agent. Veterinary Parasitology, 159, 249–252.