LEAD ACETATE INFLUENCE ON THE STRUCTURE OF LIVER OF EXPERIMENTAL ANIMALS WHEN USING WATER WITH CONTENT WITH THE CONTENT OF STEARATES OF SODIUM AND POTASSIUM

Kondratyuk V. A., Fedoriv O.Ye., Lototska O. V., Datsko T. V., Krytska G. A., Tverdohlib N. O., Sopel O. V.  LEAD ACETATE INFLUENCE ON THE STRUCTURE OF LIVER OF EXPERIMENTAL ANIMALS WHEN USING WATER WITH CONTENT WITH THE CONTENT OF STEARATES OF SODIUM AND POTASSIUM. Вода: гігієна та екологія / Water: hygiene and ecology / 2018;1-4(6):11-16. ISBN 978-617-696-121-5. DOI http://dx.doi.org/10.5281/zenodo.2567359

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LEAD ACETATE INFLUENCE ON THE STRUCTURE OF LIVER OF EXPERIMENTAL ANIMALS WHEN USING WATER WITH CONTENT WITH THE CONTENT OF STEARATES OF SODIUM AND POTASSIUM

V. A. Kondratyuk, O.Ye. Fedoriv, O. V. Lototska, T. V. Datsko, G. A. Krytska,

N. O. Tverdohlib, O. V. Sopel

I. Horbachevsky Ternopil State Medical University

Among the lead compounds, one of the most common are lead acetate, which is considered one of the most toxic anthropogenic environmental pollutants that have a polytropic effect and refers to extremely dangerous general-poisonous heavy metals (substances of the 1st class of danger), possessing the ability to accumulate their total content) in the body. Sodium and potassium stearates are not toxic (LD50> 10,000 mg / kg). They are widely included in many types of solid deodorants, rubber, latex inks, inks, and are components of some nutritional supplements and flavors that are used in the pharmaceutical industry.

The purpose of the study was to investigate the effect on the body of warm-blooded animals of lead acetate against the background of water use of animals with potassium stearate and sodium stearate.

Materials and methods of research. Experiments were carried out on four groups of white female rats weighing 150-200 g, 7 animals per group. The first (control) and the second group of animals consumed dechlorinated water from the urban water supply. The third and fourth groups of animals consumed water correspondingly with the content of sodium stearates and potassium stearate in a dose of 1/250 LD50. After 40 days of administration of the specified waters to animals of the second, third and fourth groups, lead acetate were injected orally at a dose of 1/250 of LD50. After three days, the animals were withdrawn from the experiment. The model of the experiment was a white rat liver. The liver particles were fixed in formalin, paraffin blocks were prepared, histological preparations stained with hematoxylin and eosin.

Research results. It was found that lead acetate against the background of drinking water showed a pronounced effect, in particular causing changes in the structure of the hepatic lobe and hepatocytes, deformation of cells, minor expansion and filling of erythrocytes of central veins. The sinusoids were not visualized. In separate cells, against the background of destructive changes of the cytoplasm, signs of karyopicnosis and cariolysis characterized the nucleus. There was an increase in bile duct.

When introducing lead acetate against the background of water intake with sodium stearate, the central veins and sinusoids of the centrolobular zones remained elevated and full-blooded. Microphage activity was moderate.

When introducing lead acetate against the background of water intake with potassium stearate, changes in the lobular structure of the liver parenchyma, disturbances in the beam organization of hepatocytes throughout the size of the lobe were observed. In separate cells, against the background of destructive changes in the cytoplasm, the nuclei had signs of karyopicnosis and carriolysis, and the bile ducts were somewhat expanded.

With the combined action of lead acetate against the background of water with sodium stearates and potassium, changes in the structure of the liver were less pronounced than when exposed to water with lead acetate in a dose of 1/250 LD50.

Keywords: water, lead acetate, sodium stearate,

potassium stearate, liver.