Published February 10, 2026
| Version v1
Journal article
Open
Development of a Tool for High-Efficiency, Markerless and Iterative Genome Editing in Shouchella clausii
Authors/Creators
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1.
University of Bari Aldo Moro
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2.
Danmarks Tekniske Universitetet
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3.
Chalmers University of Technology
Description
This study reports the development of a high-efficiency, markerless and iterative genome editing tool for Shouchella clausii, a Gram-positive bacterium with applications in biotherapeutics and biotechnology. The authors designed a versatile genetic platform based on a temperature-sensitive shuttle vector compatible with Golden Gate assembly, enabling precise and scarless modifications that greatly expand the genetic engineering capabilities in S. clausii and related species.
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Microbial Biotechnology - 2026 - Cappella - Development of a Tool for High‐Efficiency Markerless and Iterative Genome.pdf
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Additional details
Related works
- Is supplemented by
- Dataset: 10.5281/zenodo.17404388. (DOI)
- Is variant form of
- Journal article: 10.1101/2025.09.09.675032 (DOI)
Funding
References
- Development of a Tool for High-Efficiency, Markerless and Iterative Genome Editing in Shouchella clausii. Cappella C, Jers C, Ninivaggi L, Bettiga M, Mijakovic I, Agrimi G, Scarcia P. Microb Biotechnol. 2026 Feb;19(2):e70287. doi: 10.1111/1751-7915.70287. PMID: 41662148