Published December 20, 2025 | Version 1.0
Dataset Open

ESKAPEE BLAST nucleotide database (rMAP-2.0)

Authors/Creators

Description

This record provides a pre-built BLAST nucleotide database archive for the ESKAPEE bacterial pathogens used by rMAP-2.0. The archive contains a complete makeblastdb output directory (index and auxiliary files) so users can run blastn queries without rebuilding the database locally.

Contents

  • eskapee_db.tar.gz — compressed tar archive containing the BLAST database files (e.g., .nsq, .nin, .nhr, and related files) under eskapee_db/

  • eskapee_db.tar.gz.sha256 — SHA-256 checksum for integrity verification

How the database was built
The database was generated from a curated FASTA file of ESKAPEE bacterial sequences using NCBI BLAST+:

 
makeblastdb \ -in eskapee_db.fasta \ -dbtype nucl \ -parse_seqids \ -max_file_sz 3000000000 \ -out eskapee_db/eskapee_db

Download and unpack

 
# download from Zenodo and verify checksum sha256sum -c eskapee_db.tar.gz.sha256 # unpack tar -xzvf eskapee_db.tar.gz

Example usage
After unpacking, the database prefix is:

  • eskapee_db/eskapee_db

Example blastn query:

 
blastn -query query.fasta -db eskapee_db/eskapee_db -outfmt 6 -max_target_seqs 10 -evalue 1e-10 > blast_hits.tsv

Intended use
This database archive is intended to support reproducible, rapid local BLAST-based screening within the rMAP-2.0 workflow and related microbial genomics analyses, especially in settings where rebuilding large databases is time-consuming or bandwidth-limited.

Versioning
This Zenodo record corresponds to version of the ESKAPEE BLAST database used in rMAP-2.0. Updated databases will be released as new versions on Zenodo.

Project repository
The rMAP-2.0 code and documentation are available on GitHub: [GitHub repo link / rMAP-2.0] (add as a “Related identifier” in Zenodo).

Checksum
SHA-256 checksum is provided in eskapee_db.tar.gz.sha256 and should be used to validate file integrity after download.

Files

Files (18.3 GB)

Name Size Download all
md5:253626f5c9fd30784fa2447214d63619
18.3 GB Download
md5:79f1617317be510c69bfe575f981ed13
112 Bytes Download

Additional details

Identifiers

Other
https://gmboowa.github.io/rMAP-2.0/

Related works

Describes
Other: https://gmboowa.github.io/rMAP-2.0/ (URL)

Dates

Created
2025-12-20

Software

Repository URL
https://gmboowa.github.io/rMAP-2.0/

References