Stereo-seq data of JEV-infected mouse brains

The molecular mechanisms through which Japanese encephalitis virus (JEV) crosses the BBB and induces encephalitis remain poorly understood. In this study, we employed Stereo-seq technology to conduct a detailed characterization of the spatiotemporal transcriptional dynamics in the mouse brain after JEV infection. We inoculated BALB/c mice with 10^6 PFU of JEV in 200 μl of phosphate-buffered saline (PBS) or 200 μl of PBS in the mock-treated group. On the 3rd, 5th, and 7th day post inoculation (dpi), the mouse brains (n = 3) were collected for Stereo-seq experiment. Our findings reveal the cellular and molecular mechanisms of key pathogenic processes, including viral infection, BBB disruption, immune cell activation and recruitment, and cell death, thereby providing new targets for JE prevention and treatment.