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Published October 14, 2025 | Version 3.6.0
Software Open

nf-core/sarek: Sarek 3.6.0 - Kvikkjokk

  • 1. Seqera
  • 2. Dep. of Molecular Medicine (MOMA)
  • 3. @seqeralabs
  • 4. Fondazione Human Technopole
  • 5. Boehringer Ingelheim
  • 6. University of Tuebingen
  • 7. University of Pavia
  • 8. CoSyne Therapeutics
  • 9. INCLIVA
  • 10. Exscientia
  • 11. @CenterForMedicalGeneticsGhent
  • 12. INSERM
  • 13. ZS Associates
  • 14. UW
  • 15. SciLifeLab
  • 16. @biosharp-dotnet
  • 17. Seqera Labs
  • 18. Center for Medical Genetics Ghent
  • 19. Washington University

Description

What's Changed

Main new features

🧬 Lots of improvements around UMI support (#1937, @SPPearce) ⏩ Parabricks support (#1804, @famosab) 🔍 Muse (#1744, @famosab) and Sentieon TNScope (#1934, @SPPearce) 📜 MSISensor2 (#1965, @maxulysse) 🦖 Varlociraptor (#1940, @famosab) ◀️ Normalization of variants (#1770, @Patricie34 & @maxulysse) 🖖 bbsplit (#1983, @apsteinberg, @tobsecret & @FriederikeHanssen)

Changed

  • Version updates:
    • VEP cache updated to v115.2
    • SnpEff updated to v5.3.0a (SnpEff cache downgraded to 99 since 105 is not available at the moment)
    • GATK4 to 4.6.1.0
    • DeepVariant to 1.9.0
    • Freebayes to 1.3.10 with QUAL filtering
  • Workflow improvements:
    • Enhanced metro map
    • Doubled default process time
    • Improved sample validation
  • Complete test migration:
    • All pytest tests migrated to nf-test framework for better testing infrastructure

Fixed

New Parameters

  • --freebayes_filter
  • --msisensor2_models
  • --sentieon_consensus
  • --umi_base_skip
  • --umi_in_read_header
  • --umi_length
  • --umi_location
  • --umi_tag
  • --varlociraptor_chunk_size
  • --varlociraptor_scenario_germline
  • --varlociraptor_scenario_somatic
  • --varlociraptor_scenario_tumor_only

Full Changelog: 3.5.1...3.6.0

Files

nf-core/sarek-3.6.0.zip

Files (15.4 MB)

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md5:61f59280e3341c855c0328b8ce69dc2e
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Additional details

Related works

Is supplement to
Software: https://github.com/nf-core/sarek/tree/3.6.0 (URL)

Software