Published August 22, 2025
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Table 4 in Ray spider rush: Fast-tracking integrative taxonomy in Panama's cloud forests
Creators
- 1. Departamento de Ecologia e Biologia Evolutiva (DEBE), Universidade Federal de São Carlos (UFSCar), campus São Carlos, Rodovia Washington Luís, km 235, CEP 13565 - 905, São Carlos, SP, Brazil. & División Aracnología, Museo Argentino de Ciencias Naturales, Av. Angel Gallardo 470, C 1405 DJR Buenos Aires, Argentina.
- 2. División Aracnología, Museo Argentino de Ciencias Naturales, Av. Angel Gallardo 470, C 1405 DJR Buenos Aires, Argentina.
- 3. Department of Biodiversity and Conservation, Mediterranean Institute for Advanced Studies, IMEDEA, Balearic Islands, Spain.
- 4. Department of Biological Sciences, The George Washington University, Washington, DC 20052, USA.
- 5. Departament de Biologia Evolutiva, Ecologia i Ciències Ambientals & Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, Spain.
Description
Table 4. Primer sequences, source and annealing temperatures for mitochondrial cytochrome c oxydase subunit I (COI).
| Forward | Sequence | Reference | Annealing temperature |
|---|---|---|---|
| C1-J-1490 / LCO-1490 | 5’-GGTCAACAAATCATAAAGATATTGG-3’ | Folmer et al. (1994) | 42–45°C |
| C1-N-2191 / Nancy | 5’-CCCGGTAAAATTAAAATATAAACTTC-3’ | Folmer et al. (1994) | 42–45°C |
| LepF1 | 5’-ATTCAACCAATCATAAAGATATTGG-3’ | Hebert et al. (2004) | 40°C / 51°C |
| M13F (-21) | 5’-TGTAAAACGACGGCCAGT-3’ | Messing (1983) | 40°C / 51°C |
| Reverse | Sequence | Reference | Annealing temperature |
| C1-N-2198 / HCO-2198 | 5’-TAAACTTCAGGGTGACCAAAAAATCA-3’ | Folmer et al. (1994) | 42–45°C |
| C1-N-2198 / HCO-2198 | 5’-TAAACTTCAGGGTGACCAAAAAATCA-3’ | Folmer et al. (1994) | 42–45°C |
| LepR1 | 5’-TAAACTTCTGGATGTCCAAAAAATCA-3’ | Hebert et al. (2004) | 40°C / 51°C |
| M13R (-27) | 5’-CAGGAAACAGCTATGAC-3’ | Messing (1983) | 40°C / 51°C |
Notes
Published as part of Labarque, Facundo M., Piacentini, Luis N., Pons, Joan, Hormiga, Gustavo, Arnedo, Miquel A. & Ramírez, Martín J., 2025, Ray spider rush: Fast-tracking integrative taxonomy in Panama's cloud forests, pp. 22 in European Journal of Taxonomy 1010 on page 22, DOI: 10.5852/ejt.2025.1010.3021, http://zenodo.org/record/17202080
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- Is part of
- Journal article: 10.5852/ejt.2025.1010.3021 (DOI)
- Journal article: urn:lsid:plazi.org:pub:FFF0FFBCB55C8C56FFF0FFD5FF88FA13 (LSID)
- Journal article: http://publication.plazi.org/id/FFF0FFBCB55C8C56FFF0FFD5FF88FA13 (URL)
- Journal article: http://zenodo.org/record/17202080 (URL)
- Journal article: http://zoobank.org/90524F49-7BA2-4B8A-9BE3-450CB77A31A0 (URL)
References
- Folmer O., Black M., Hoeh W., Lutz R. & Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology Biotechnology 3 (5): 294-299.
- Hebert P. D. N., Penton E. H., Burns J. M., Janzen D. H. & Hallwachs W. 2004. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. PNAS 101 (41): 14812-14817. https://doi.org/10.1073/pnas.0406166101
- Messing J. 1983. [2] New M 13 vectors for cloning. Methods in Enzymology 101: 20-78. https://doi.org/10.1016/0076-6879(83)01005-8