Published September 4, 2025 | Version v2
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Test Data for Bulk RNA-seq Pipeline 2025

  • 1. ROR icon St. Jude Children's Research Hospital

Description

Here are the test data, containing six samples of different conditions, for bulk RNA-seq quantification pipeline 2025.

  Format Library Type Phred Encoding Species Number of Reads Preparation
sample1 FASTQ Paired-end Phred+33 human 19,410,373 Downsampled from real data
sample2 FASTQ Single-end Phred+33 mouse 16,005,450 Downsampled from real data
sample3 FASTQ, multiple lanes Paired-end Phred+33 human 19,410,373 Split by lane from sample1
sample4 FASTQ, multiple lanes Single-end Phred+33 mouse 16,005,450 Split by lane from sample2
sample5 BAM/SAM (to genome) Paired-end Phred+33 human 13,856,075 Downsampled from real data
sample6 BAM/SAM (to transcriptome) Single-end Phred+33 mouse 13,533,162 Downsampled from real data

Files

sample1.zip

Files (8.9 GB)

Name Size Download all
md5:cc4c16fdcb152c2d2518e94facf70d98
2.0 GB Preview Download
md5:6128ae80588cf407591407641fe18c52
448.7 MB Preview Download
md5:33ccf0ef0f3dc3db22ae56b95dddde57
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md5:23b087a4d488e98a3fd3ee0b1915879c
462.2 MB Preview Download
md5:e422326eb5b6cb6ead4c918166f53e6f
3.0 GB Preview Download
md5:29c26f93b03879d35902909b4936c98a
978.7 MB Preview Download

Additional details

Dates

Created
2025-09-09

Software

Repository URL
https://github.com/jyyulab/bulkRNAseq_quantification_pipeline
Programming language
Perl, R, Shell