clockgene/Sladek-et-al.-PLoS-Biol-2025: Sladek et al. PLoS Biol 2025

data and script for analysis and figures submitted to Plos Biol 5.9.2025

raw metabolomics and RT qPCR data are in the csv file - contains not only SCN, but also PLS and LIV metabolites. Differential expresssion, clustering and rhythm detection needs to be done separately and merged manually with processed data. External algorithms used: eJTK (https://biodare2.ed.ac.uk/), CircaCompare (modified version included in the script), deseq2 and clustering (RNAlysis). Individual figures are generated as separate svg files and combined together using Corel Draw or InkScape.

see the manuscript methods for details:

Maternal food-derived signals oscillate in the fetal suprachiasmatic nuclei before its circadian clock develops

Martin Sládek 1, Pavel Houdek 1, Tomáš Čajka 2, Alena Sumová 1

1Laboratory of Biological Rhythms, Institute of Physiology of the Czech Academy of Sciences 2Laboratory of Translational Metabolism, Institute of Physiology of the Czech Academy of Sciences