Published September 30, 2024
| Version v1
Journal article
Open
How to best utilise molecular data to support new taxa - a practical guide in less than 5000 words
Creators
Description
M., MÖller, H., Atkins, Nishii, K. (2024): How to best utilise molecular data to support new taxa - a practical guide in less than 5000 words. Rheedea 34 (3): 165-182, DOI: 10.22244/rheedea.2024.34.03.01
Files
source.pdf
Files
(6.0 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:3066f05518583ede86163032b24ad6d9
|
6.0 MB | Preview Download |
Linked records
Additional details
Identifiers
- LSID
- urn:lsid:plazi.org:pub:3066F0551858FFDE86163032B24AFFD9
References
- ATKINS H.J., BRAMLEY G.L.C., NISHII K., MOLLER M., OLIVAR J.E.C., KARTONEGORO A. & M. HUGHES 2021. Sectional polyphyly and morphological homoplasy in Southeast Asian Cyrtandra (Gesneriaceae): consequences for the taxonomy of a mega-diverse genus. Plant Systematics and Evolution 307: 60. https://doi.org/10.1007/ s00606-021-01784-x
- BARKER F.K. & F.M. LUTZONI 2002. The utility of the incongruence length difference test. Systematic Biology 51(4): 625-637. https://doi. org/10.1080/10635150290102302
- BAUM D.A. & S.D. SMITH 2012. Tree thinking: an introduction to phylogenetic biology. Roberts and Co., Greenwood Village, CO.
- BOLUDA C.G., CHRISTE C., NACIRI Y., & L. GAUTIER 2022. A 638-gene phylogeny supports the recognition of twice as many species in the Malagasy endemic genus Capurodendron (Sapotaceae). Taxon 71(2): 360-395. https://doi.org/10.1002/tax.12676
- CHEN W.H., MOLLER M., SHUI Y.M., WANG H., YANG J.B. & G.Y. LI 2014. Three new species of Petrocodon (Gesneriaceae), endemic to the limestone areas of Southwest China, and preliminary insights into the diversification patterns of the genus. Systematic Botany 39(1): 316-330. https://doi. org/10.1600/036364414X678189
- CLARKE C.B. 1883. Cyrtandreae. In: DE CANDOLLE A. & C. DE CANDOLLE (eds.), Monographiae Phanerogamarum. Volume 5/1. Masson G., Paris, pp. 1-303.
- CUNNINGHAM C.W. 1997. Can three incongruence tests predict when data should be combined?
- DE QUEIROZ 2007. Species concepts and species delimitation. Systematic Biology 56(6): 879-886. https://doi.org/10.1080/10635150701701083
- DE VILLIERS M.J., PIRIE M.D., HUGHES M., MOLLER M., EDWARDS T.J. & D.U.B. BELLSTEDT 2013. An approach to identify putative hybrids in the 'coalescent stochasticity zone', as exemplified in the African plant genus Streptocarpus (Gesneriaceae). New Phytologist 198(1): 284-300. https://doi.org/10.1111/nph.12133
- DENDUANGBORIPANT J. & Q.C.B. CRONK 2000. High intraindividual variation in internal transcribed spacer sequences in Aeschynanthus (Gesneriaceae): implications for phylogenetics. Proceedings of the Royal Society B, Biological Sciences 267(1451): 1407-1415. https://doi.org/10.1098/rspb.2000.1157
- DOUZERY E.J.P., PRIDGEON A.M., KORES P., LINDERH.P., KURZWEILH.& M.W. CHASE 1999. Molecular phylogenetics of Diseae (Orchidaceae): a contribution from nuclear ribosomal ITS sequences. American Journal of Botany 86(6): 887-899. https:// doi.org/10.2307/2656709
- DOYLE J.J. & J.L. DOYLE 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
- DROUIN G., DAOUD H. & J. XIA 2008. Relative rates of synonymous substitutions in the mitochondrial, chloroplast and nuclear genomes of seed plants. Molecular Phylogenetics and Evolution 49(3): 827-831. https://doi.org/10.1016/j.ympev.2008.09.009
- DUMINIL J. & M. DI MICHELE 2009. Plant species delimitation: a comparison of morphological and molecular markers. Plant Biosystems 143(3): 528-542. https://doi.org/10.1080/11263500902722964
- FARRIS J., KALLERSJO S.M., KLUGE A.G. & C. BULT 1995a. Constructing a significance test for incongruence. Systematic Biology 44(4): 570-572. https://doi.org/10.2307/2413663
- FARRIS J., KALLERSJO S.M., KLUGE A.G. & C. BULT 1995b.Testingsignificanceofincongruence Cladistics . 10: 315-319. https://doi.org/10.1111/j.1096-0031.1994. tb00181.x
- FELSENSTEIN J. 2004. Inferring phylogenies. Sinauer Associates, Sunderland, Massachusetts.
- G., VANGESTEL C., VIRGILIO M. & P.M. HOLLINGSWORTH 2023. Developing the protocol infrastructure for DNA sequencing natural history collections. Biodiversity Data Journal 11: e102317. https:// doi.org/10.3897/BDJ.11.e102317
- GREINER S., SOBANSKI J. & R. BOCK 2015. Why are most organelle genomes transmitted maternally? BioEssays 37: 80-94. https://doi.org/10.1002/bies.201400110
- GROSSER M.R., SITES S.K., MURATA M.M., LOPEZ Y., CHAMUSCO K.C., LOVE HARRIAGE K., GROSSER J.W., GRAHAMJ.H.,GMITTERF.G.JR.& C.D. CHASE 2023. Plant mitochondrial introns as genetic markers - conservation and variation. Frontiers in Plant Sciences 14: 1116851. https://doi.org/10.3389/fpls.2023.1116851
- GUO J., PAN B., LIU J., XU W.B. & K.F. CHUNG 2015. Three new species of Primulina (Gesneriaceae) from limestone karsts of China based on morphological and molecular evidence. Botanical Studies 56(34): 1-13. https://doi.org/10.1186/s40529-015-0115-5
- HALE M.L., BURG T.M. & T.E. STEEVES 2012. Sampling for microsatellite-based population genetic studies: 25 to 30 individuals per population is enough to accurately estimate allele frequencies. PLoS ONE 7: e45170. https:// doi.org/10.1371/journal.pone.0045170
- HANCE H.F. 1883. New Chinese Cyrtandreae. Journal of Botany 21: 165-170.
- HASSANPOUR S., MOAZZENI H., SONBOLI A., HOSEINI S., PIRANI A., JOHARCHI M.R. & C. OBERPRIELER 2023. Molecular and morphological data reveal a new species of Sclerorhachis (Compositae, Anthemideae) and the reassessment of another species of the genus. Plant Systematics and Evolution 309: 10. https://doi.org/10.1007/ s00606-022-01840-0
- HIPP A.L., HALL J.C. & K.J. SYTSMA 2004. Congruence versus phylogenetic accuracy:revisiting the incongruence length difference test. Systematic Biology 53(1): 81-89. https://doi.org/10.1080/10635150490264752
- HONG X., KEENE J., QIU Z.J. & F. WEN 2019. Primulina anisocymosa (Gesneriaceae), a new species with a unique inflorescence structure from Guangdong, China. PeerJ 7: e6157. https://doi.org/10.7717/peerj.6157
- HU J., ZHANG J.Y., HE H., YU D.X., JIANG H., LIU Q. & F. WEN 2023. Oreocharis oriolus, a new species of Gesneriaceae in a sclerophyllous oak community from Yunnan, Southwest China. Ecology and Evolution 13(6): e10174. https://doi.org/10.1002/ece3.10174
- HUANGJ.,XIANGX.G.,LUY.B.,PANB.&Q.ZHANG2017. Hemiboea pterocaulis comb. et stat. nov. (Gesneriaceae), a new critically endangered species segregated from H. subcapitata. Nordic Journal of Botany 2018: e01468. https:// doi.org/10.1111/njb.01468
- JANEESHA A.P., RANASINGHE S., MOLLER M., DILNA P.K. & S. NAMPY 2023. Revision of Rhynchoglossum (Gesneriaceae) in South India and Sri Lanka, reinstating R. scabrum based on a multi-sample molecular-morphological approach. Plant Systematics and Evolution 309: 26. https://doi.org/10.1007/s00606-023-01858-y
- JOLY S., MCLENACHAN P.A. & P.J. LOCKHART 2009. A statistical approach for distinguishing hybridization and incomplete lineage sorting. American Naturalist 174(2): E54-E70. https://doi.org/10.1086/600082
- KANTHRAJ A.S., RANA T.S., TIWARI A. & K.N. NAIR 2023. Taxonomic reappraisal of Henckelia hookeri and H. collegii-sancti-thomasii (Gesneriaceae): two little-known endemic species of the Eastern Himalaya in India. Kew Bulletin 78: 625-640. https://doi.org/10.1007/s12225- 023-10090-3
- KATOH K., ROZEWICKI J. & K.D. YAMADA 2019. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20(4): 1160-1166. https://doi. org/10.1093/bib/bbx108
- KIM J.Y., ROHLF F.J. & R.R. SOKAL 1993. Accuracy of phylogenetic estimation using the Neighbor- Joining method. Evolution 47(2): 471-486. https://doi. org/10.2307/2410065
- KNOWLES L.L.& B.C.CARSTENS2007.Delimiting species without monophyletic gene trees. Systematic Biology 56(6): 887-895. https://doi.org/10.1080/10635150701701091
- KONG H.H., CONDAMINE F.L., YANG L.H., HARRIS A.J., FENG C., WEN F. & M. KANG 2022. Phylogenomic and macroevolutionary evidence for an explosive radiation of a plant genus in the Miocene. Systematic Biology 71(3): 589-609. https://doi.org/10.1093/sysbio/syab068
- LEMEY P., SALEMI M. & A.M. VANDAMME 2009. The phylogenetic handbook second edition: a practical approach to phylogenetic analysis and hypothesis testing. Cambridge University Press, Cambridge.
- LI P.W., LIU F.P., HAN M.Q., SMITH J.F. & Y.Z. WANG 2022. Molecular and morphological evidence supports the inclusion of Deinostigma into Metapetrocosmea (Gesneriaceae). Annals of the Missouri Botanical Garden 107: 447-466. https://doi.org/10.3417/2022767
- LIUY., XUW.B.& Y.S.HUANG 2011.Primulina guangxiensis sp. nov. (Gesneriaceae) from a karst cave in Guangxi, China. Nordic Journal of Botany 29: 682-686. https://doi. org/10.1111/j.1756-1051.2011.01089.x
- LUIKART G. & J. CORNUET 1998. Empirical evaluation of a test for identifying recently bottlenecked populations fromallelefrequencydata.ConservationBiology 12(1):228- 237. https://doi.org/10.1111/j.1523-1739.1998.96388.x
- LUU H.T., HSIEH C.L., CHUANG C.R., CHEN C.W., TRAN N.T., VU N.L. & K.F. CHUNG 2023.Langbiangia, a new genus of Gesneriaceae endemic to Langbiang Plateau, southern Vietnam and a taxonomic endeavor to achieve key targets of the post-2020 global biodiversity framework. PLoS ONE 18(5): e0284650. https://doi. org/10.1371/journal.pone.0284650
- MCKAIN M.R., JOHNSON M.G., URIBE-CONVERS S. EATON D. & Y. YANG 2018. Practical considerations for plant phylogenomics.Applications in Plant Sciences 6(3): e1038. https://doi.org/10.1002/aps3.1038
- MEIER R. & B. WHEELER 2008. DNA sequences in taxonomy, opportunities and challenges. The New Taxonomy 65-127.
- MEYER C.P.& G.PAULAY 2005.DNA barcoding:error rates based on comprehensive sampling. PLOS Biology 3(12): e422 https://doi.org/10.1371/journal.pbio.0030422
- MOGENSEN H.L. 1996. The hows and whys of cytoplasmic inheritance in seed plants. American Journal of Botany 83: 383-404. https://doi.org/10.1002/j.1537-2197.1996. tb12718.x
- MOLLER M. 2019. Species discovery in time: an example from Gesneriaceae in China.Guangxi Sciences 26(1): 1-16. https://doi.org/10.13656/j.cnki.gxkx.20190307.002
- MOLLER M. & Q.C.B. CRONK 1997. Origin and relationships of Saintpaulia (Gesneriaceae) based on ribosomal DNA internal transcribed spacer (ITS) sequences. American Journal of Botany 84(8): 956-965. https://doi.org/10.2307/2446286
- MOLLER M. & Q.C.B. CRONK 2001. Evolution of morphological novelty: a phylogenetic analysis of growth patterns in Streptocarpus (Gesneriaceae). Evolution 55(5): 918-929. https://doi.org/10.1111/j.0014-3820.2001. tb00609.x
- MOLLER M. & J.L. CLARK 2013. The state of molecular phylogenetic work in the family Gesneriaceae: a review. Selbyana 31(2): 95-125. https://www.jstor.org/ stable/24894284
- MOLLER M., KOKUBUGATA G. & K. JONG 2008. Aspects of genome evolution in Gesneriaceae: patterns of 45S-nrDNA site evolution based on evidence from fluorescent in-situ hybridization (FISH). International Journal of Plant Sciences 169(5): 667-676. https://doi. org/10.1086/533609
- MOLLER M., PFOSSER M., JANG C.G., MAYER V., CLARK A., HOLLINGSWORTH M.L., BARFUSS M.H.J., WANG Y.Z., KIEHN M. & A. WEBER 2009. A preliminary phylogeny of the "didymocarpoid Gesneriaceae" based on three molecular data sets: incongruence with available tribal classifications.
- American Journal of Botany 96(5): 989-1010. https://doi. org/10.3732/ajb.0800291
- MOLLER M., FORREST A., WEI Y.G. & A. WEBER 2011a. A molecular phylogenetic assessment of the advanced Asiatic and Malesian didymocarpoid Gesneriaceae with focus on non-monophyletic and monotypic genera. Plant Systematics and Evolution 292: 223-248. https://doi. org/10.1007/s00606-010-0413-z
- MOLLER M., MIDDLETON D.J., NISHII K., WEI Y.G., SONTAG S. & A. WEBER 2011b. A new delineation for Oreocharis incorporating an additional ten genera of Chinese Gesneriaceae. Phytotaxa 23: 1-36. https://doi. org/10.11646/phytotaxa.23.1.1
- MOLLER M., CHEN W.H., SHUI Y.M., ATKINS H. & D.J. MIDDLETON 2014. A new genus of Gesneriaceae in China and the transfer of Briggsia species to other genera. Gardens' Bulletin Singapore 66(2):195-205. https://biostor. org/reference/217698
- MOLLER M., WEI Y.G., WEN F., CLARK J.L. & A. WEBER 2016. You win some you lose some: updated generic delineations and classification of Gesneriaceae - implications for the family in China. Guihaia 36(1): 44- 60. https://doi.org/10.11931/guihaia.gxzw201512015
- MOLLER M, LIU J., LI Y., LIE J.H., YEA L.J., MILL R., THOMAS P., LI D.Z. & L.M. GAO 2020. Repeated intercontinental migrations and recurring hybridizations characterise the evolutionary history of yew (Taxus L.). Molecular Phylogenetics and Evolution 153: e106952. https://doi.org/10.1016/j.ympev.2020.106952
- NEI M. & S. KUMAR 2000. Molecular evolution and phylogenetics. Oxford University Press, Oxford.
- NISHII K., HUGHES M., BRIGGS M., HASTON E., CHRISTIE F., DEVILLIERS M.J., HANEKOM T., ROOS W.G., BELLSTEDT D.U. & M. MOLLER 2015. Streptocarpus redefined to include all Afro-Malagasy Gesneriaceae: molecular phylogenies prove congruent with geography and cytology and uncovers remarkable morphological homoplasies. Taxon 64(6): 1243-1274. https://doi.org/10.12705/646.8
- NOPPORNCHAROENKUL N., SOONTORNCHAINAK- SAENG P., JENJITTIKUL T., CHUENBOONNGARM N. & U. VIBOONJUN 2016. Kaempferia simaoensis (Zingiberaceae), a new record for Thailand: evidence from nuclear ITS2 sequence analyses. Thai Journal of Botany 8: 81-91.
- OGUTCEN E., CHRISTE C., NISHII K., SALAMIIN N., MOLLER M. & M. PERRET 2021. Phylogenomics of Gesneriaceae using targeted capture of nuclear genes. Molecular Phylogenetics and Evolution 157: 107068. https:// doi.org/10.1016/j.ympev.2021.107068
- PAGE R.D.M. & E.C. HOLMES 1998. Molecular evolution: a phylogenetic approach. Wiley-Blackwell, Cambridge.
- PATON J. 2023. What's in an NOR? Nuclear ribosomal DNA variation in Aeschynanthus (Gesneriaceae) - what can be revealed with genome skimming? MSc thesis (unpublished), The University of Edinburgh.
- PELSER P.B., ABBOTT R.J., COMES H.P., MILTON J.J., MOLLER M., LOOSELEY M.E., CRON G.V., BARCELONA J.F., KENNEDY A.H., WATSON L.E., BARONE R., HERNANDEZ F. & J.W. KADEREIT 2012. The genetic ghost of an invasion past: colonization and extinction revealed by historical hybridization in Senecio. Molecular Ecology 21(2): 369-387. https://doi. org/10.1111/j.1365-294X.2011.05399.x
- PENNINGTON R.T. & M. LAVIN 2016. The contrasting nature of woody plant species in different neotropical forest biomes reflects differences in ecological stability. New Phytologist 210(1): 25-37. https://doi.org/10.1111/ nph.13724
- PUGLISI C., WEI Y.G., NISHII K. & M. MOLLER 2011. Oreocharis × heterandra (Gesneriaceae): a natural hybrid from the Shengtangshan Mountains, Guangxi, China. Phytotaxa 38: 1-18. https://doi.org/10.11646/ phytotaxa.38.1.1
- PUGLISI C., YAO, T.L., MILNE R., MOLLER M. & D.J. MIDDLETON 2016. Generic recircumscription in the Loxocarpinae (Gesneriaceae), as inferred by phylogenetic and morphological data. Taxon 65(2): 277-292. https:// doi.org/10.12705/652.5
- QIN H.T., MOLLER M., MILNE R., LUO Y.H., ZHU G.F., LI D.Z., LIU J. & L.M. GAO 2023. Multiple paternally inherited chloroplast capture events associated with Taxus speciation in the Hengduan Mountains. Molecular Phylogenetics and Evolution 189: 1-12. https://doi. org/10.1016/j.ympev.2023.107915
- RANASINGHE S.W. 2017. Molecular species delimitation, taxonomy and biogeography of Sri Lankan Gesneriaceae. PhD thesis (Unpublished), The University of Edinburgh.
- RANASINGHE S.W., MILNE R., JAYASEKARA R., RUBASINGHE S. & M. MOLLER 2016. Henckelia wijesundarae (Gesneriaceae), a new endemic species from Sri Lanka and lectotypification of Henckelia walkerae and Chirita walkerae var. parviflora. Willdenowia 46: 213-224. https://doi.org/10.3372/wi.46.46202
- RANASINGHE S.W., NISHII K., MOLLER M., ATKINS H.J., CLARK J.L., PERRET M. KARTONEGORO A., GAO L.M., MIDDLETON D.J. & R.I. MILNE 2024. Biogeographic history of the pantropical family Gesneriaceae with a focus on the Indian plate and diversification through the Old World. Frontiers of Biogeography 16(2): e61637. https://doi.org/10.21425/
- ROSE J.P., TOLEDO C.A.P., MORIARTY LEMMON E., LEMMON A.R. & K.J. SYSTMA 2020. Out of sight, out of mind: widespread nuclear and plastid-nuclear discordance in the flowering plant genus Polemonium (Polemoniaceae) suggests widespread historical gene flow despite limited nuclear signal. Systematic Biology 70(1): 162-180. https://doi.org/10.1093/sysbio/syaa049
- SARKINEN T., STAATS M., RICHARDSON J.E., COWAN R.S. & F.T. BAKKER 2012. How to open the treasure chest? Optimising DNA extraction from herbarium specimens.PLoS One 7(8): e43808.https://doi. org/10.1371/journal.pone.0043808
- SHAW J., LICKEY E.B., BECK J.T., FARMER S.B., LIU W.S., MILLER J., SIRIPUN K.C., WINDER C.T., SCHILLING E.E. & R.L. SMALL 2005. The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. American JournalofBotany 92(1):142-166.https://doi.org/10.3732/ ajb.92.1.142
- SHAW J., LICKEY E.B., SCHILLING E.E. & R.L. SMALL 2007. Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms: the tortoise and the hare III. American Journal of Botany 94(3): 275-288. https://doi. org/10.3732/ajb.94.3.275
- SHAW J., SHAFER H.L., LEONARD O.R., KOVACH M.J., SCHORR M. & A.B. MORRIS 2014. Chloroplast DNA sequence utility for the lowest phylogenetic and phylogeographic inferences in angiosperms: the tortoise and the hare IV. American Journal of Botany 101(11): 1987-2004. https://doi.org/10.3732/ajb.1400398
- SMALL R.L., RYBURN J.A., CRONN R.C., SEELANAN T. & J.F. WENDEL 1998. The tortoise and the hare: choosing between noncoding plastome and nuclear Adh sequences for phylogeny reconstruction in a recently diverged plant group. American Journal of Botany 85(9): 1301-1315. https://doi.org/10.2307/2446640
- SOLTIS D.E. & R.K. KUZOFF 1995. Discordance between nuclear and chloroplast phylogenies in the Heuchera group (Saxifragaceae). Evolution 49(4): 727-742. https:// doi.org/10.1111/j.1558-5646.1995.tb02309.x
- SPANGLER R.E. & R.G. OLMSTEAD 1999. Phylogenetic analysis of Bignoniaceae based on the cpDNA gene sequences rbc L and ndh F. Annals of the Missouri Botanical Garden 86: 33-46. https://doi.org/10.2307/2666216
- STOEBEB.,HANSMANS., GOREMYKINV.,KOWALLIK K.V. & W. MARTIN 1999. Proteins encoded in sequenced chloroplast genomes: an overview of gene content, phylogenetic information and endosymbiotic gene transfer to the nucleus. In: HOLLINGSWORTH P.M., BATEMAN R.M. & R.J. GORNALL (eds), Molecular systematics and plant evolution. Taylor & Francis, London. pp. 327-352.
- SWOFFORD D.L. 1991. When are phylogeny estimates from molecular and morphological data incongrugent? In: MIYAMOTO M.M. & J. CRACRAFT (eds.), Phylogenetic analysis of DNA sequences. Oxford University Press, Oxford. pp. 295-333.
- TAN K., CHEN D.Y., SONG X.Q. & M.X. REN 2023. A new lithophilous species of Gesneriaceae, Petrocodon rubrostriatus, from the karst area of South Yunnan, China. PhytoKeys 230: 289-299.https://doi.org/10.3897/ phytokeys.230.106358
- TATENO Y., TAKEZAKI N. & M. NEI 1994. Relative efficiencies of the maximum-likelihood, neighborjoining, and maximum-parsimony methods when substitution rate varies with site. Molecular Biology and Evolution 11(2): 261-77. https://doi.org/10.1093/ oxfordjournals.molbev.a040108
- THEOBALD W.L. & D.A. GRUPE 1972. Critical notes on Ceylon plants: a new name and combinations in the Gesneriaceae. Ceylon Journal of Science, Biological Sciences 10: 70-71.
- THWAITES G.H.K. 1864. Cyrtandraceae. In: Enumeratio plantarum Zeylaniae. Dulau & Co., London. pp. 206-208.
- TYSZKA A.S., BRETZ E.C., ROBERTSON H.M., WOODCOCK-GIRARD M.D., RAMANAUSKAS K., LARSON D.A., STULL G.W. & J.F. WALKER 2023. Characterizing conflict and congruence of molecular evolution across organellar genome sequences for phylogenetics in land plants. Frontiers in Plant Sciences, Section Plant Bioinformatics 14: 2023. https://doi. org/10.3389/fpls.2023.1125107
- WANG W.C., ZHANG X.Z., GARCIA S., LEITCH A.R. & A. KOVARIK 2023. Intragenomic rDNA variation - the product of concerted evolution, mutation, or something in between? Heredity 131: 179-188. https:// doi.org/10.1038/s41437-023-00634-5
- WANG Y.Z., MAO R.B., LIU Y.LI J.M., DONG Y., LI Z.Y. & J.F. SMITH 2011. Phylogenetic reconstruction of Chirita and allies (Gesneriaceae) with taxonomic treatments. Journal of Systematics and Evolution 49(1): 50-64. https:// doi.org/10.1111/j.1759-6831.2010.00113.x
- WARD S.M. & M. JASIENIUK 2009. Review: sampling weedy and invasive plant populations for genetic diversity analysis. Weed Science 57(6): 593-602. https:// doi.org/10.1614/WS-09-082.1
- WEBER A., MIDDLETON D.J., FORREST A., KIEW R., LIM C.L., RAFIDAH A.R., SONTAG S., TRIBOUN P., WEI Y.G., YAO T.L. & M. MOLLER 2011a. Molecular systematics and remodelling of Chirita and associated genera (Gesneriaceae). Taxon 60(3): 767-790. https:// doi.org/10.1002/tax.603012
- WEBER A., WEI Y.G., PUGLISI C., WEN F., MAYER V. & M. MOLLER 2011b. A new definition of the genus Petrocodon (Gesneriaceae). Phytotaxa 23: 49-67. https:// doi.org/10.11646/phytotaxa.23.1.3
- WEN F., LI S., XIN Z.B., FU L.F., HONG X., CAI L., QIN J.Q., PAN B., PAN F.Z. & Y.G. WEI 2019. The updated plant list of Gesneriaceae in China under the new Chinese naming rules. Guangxi Science 26(1): 37-63.
- WEN F., WEI Y.G., FU L.F., XIN Z.B., LI S., HUANG Z.J., GE Y.Z. & D.C. MENG 2021. The checklist of Gesneriaceae in China. http://gccc.gxib.cn/cn/about-68. aspx
- WEN F., XIN Z.B., HONG X., CAI L., CHEN X.Y., LIANG J.J., WANG H.F., MACIEJEWSKI S., WEI Y.G. & L.F. FU 2022.Actinostephanus (Gesneriaceae), a new genus and species from Guangdong, South China. PhytoKeys 193: 89-106. https://doi.org/10.3897/phytokeys.193.80715
- WHITE T.J., BURNS T.D., LEE S.B. & J.W. TAYLOR 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.In: INNIS M.A., GELFAND D.H., SNINSKY J.J. & T.J. WHITE (eds.), PCR protocols. Academic Press, London. pp. 315-322.
- WOLFE K.H., LI W.H. & P.M. SHARP 1987. Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs. Proceedings of the National Academy of Sciences USA 84(24): 9054-9058. https://doi.org/10.1073/pnas.84.24.9054
- WORTLEY A.H., RUDALL P.J., HARRIS D.J. & R.W. SCOTLAND 2005. How much data are needed to resolve a difficult phylogeny? Case study in Lamiales. Systematic Biology 54(5): 697-709. https://doi. org/10.1080/10635150500221028
- XIAO L.Q., MOLLER M. & H. ZHU 2010. High nrDNA ITS polymorphism in the ancient extant seed plant Cycas: incomplete concerted evolution and the origin of pseudogenes. Molecular Phylogenetics and Evolution 55(1): 168-177. https://doi.org/10.1016/j.ympev.2009.11.020
- XU G.L., LIANG L.F., CHEN D.Y. JING Z.F., ZUO X.H., ZUO Z.Y. & F. WEN 2023. Primulina jiulianshanensis, a new species of Gesneriaceae from Jiangxi province, China. PhytoKeys 226: 1-16. https://doi.org/10.3897/ phytokeys.226.96351
- XU W.B., LIU Y., KONO Y., CHANG H., PENG C.I. & K.F. CHUNG 2013. Primulina cardaminifolia (Gesneriaceae), a rare new species from limestone areas in Guangxi, China. Botanical Studies 54: 19. https://doi.org/10.1186/1999- 3110-54-19
- XU W.B., MENG T., ZHANG Q., WU W.H., LIU Y. & K.F. CHUNG 2014.Petrocodon (Gesneriaceae) in the limestone karsts of Guangxi, China: three new species and a new combination based on morphological and molecular evidence. Systematic Botany 39(3): 965-974. https://doi. org/10.1600/036364414X681437
- YANG L.H., HARRIS A.J., WEN F., LI Z., FENG C., KONG H.H. & M. KANG 2023. Phylogenomic analyses reveal an allopolyploid origin of core Didymocarpinae (Gesneriaceae) followed by rapid radiation. Systematic Biology 72(5): 1064-1083. https://doi.org/10.1093/ sysbio/syad029
- YAO P.C., GAO H.Y., WEI Y.N., ZHANG J.H., CHEN X.Y. & H.Q. LI 2017. Evaluating sampling strategy for DNA barcoding study of coastal and inland halo-tolerant Poaceae and Chenopodiaceae: a case study for increased sample size. PLoS ONE 12(9): e0185311. https://doi. org/10.1371/journal.pone.0185311
- YU X.L., LI M., ZHOU J.J. & P.W. LI 2015. Petrocodon hunanensis (Gesneriaceae), a new species identified by both morphological and molecular evidence from limestone area in Hunan, China.Phytotaxa 195(1): 65-72. https://dx.doi.org/10.11646/phytotaxa.195.1.4
- ZHOU P., LI J. & M. MOLLER 2017. Secondary contact, hybridization and polyploidization add to the biodiversity in the Hengduan Mountains, exemplified by the widespread Corallodiscus lanuginosus (Gesneriaceae). Plant Systematics and Evolution 303(5): 587-602. https://doi. org/10.1007/s00606-017-1392-0
- ZICH F.A. & B. GRAY 2021. Boea resupinata Zich & B.Gray (Gesneriaceae), a new species from Cape York Peninsula, Queensland, Australia. Austrobaileya 11: 56-66. Available from: https://www.qld.gov.au/__data/assets/ pdf_file/0017/209600/zich-and-gray-boea-resupinataaustrobaileya-v11-56-66.pdf (Accessed on 16.09.2024).