NFDI4BIOIMAGE
Published September 15, 2025 | Version v1
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NFDI4BIOIMAGE Calendar September 2025

Creators

  • 1. Leibniz Institute for Neurobiology, Magdeburg, Germany
  • 2. Combinatorial NeuroImaging Core Facility, Leibniz Institute for Neurobiology, Magdeburg, Germany
  • 3. Institute for Molecular and Clinical Immunology and Service Unit Multiparametric Bioimaging and Cytometry, University of Magdeburg, Germany
  • 4. Institute of Materials, Technologies and Mechanics, University of Magdeburg, Germany

Contributors

Data curator:

  • 1. ROR icon Heinrich Heine University Düsseldorf
  • 2. ROR icon NFDI4BIOIMAGE

Description

Image from the NFDI4BIOIMAGE Calendar September 2025.

The scanning electron micrograph shows the approach of T-lymphocytes (Jurkat cells; cyan) to an antigen-presenting B cell (Raji cell; yellow) in the center. The image was taken as part of the research work of the CRC 854, which focused on molecular processes that regulate inter- and intracellular communication within the immune system.

Image Metadata (using REMBI template):

Study

Study description

Ultrastructure of the immune synapse

Study type

Research project within DFG CRC 854 (Molecular organisation of cellular communication within the immune system)

Study Component

Imaging method

Scanning Electron Microscopy

Biosample

Biological entity

Jurkat cell line E6.1 and Raji B cell lymphoma cell line

Organism

Homo sapiens

Identity

Z21_A1

Specimen

Preparation method

Cell lines were maintained in RPMI 1640 medium supplemented with 10% fetal calf serum (FCS; PAN Biotech), stable L-glutamine, penicillin (50 U/ml), and streptomycin (50 mg/ml) (Biochrom) in humidified 5% CO2 at 37°C. E6.1 cells were mixed at a 1:1 ratio with Raji B cells that had been pulsed with SEE (bacterial SAG staphylococcal enterotoxin E). After 10 min cells were plated on poly-L-lysine–covered slides at room temperature for 5 min and fixed for 10 min in PBS (pH 7.4) containing 1.5% PFA and 0.025% glutaraldehyde. Cryo-drying by critical point dryer (Leica EM CPD300) followed by sputtering with gold.

Signal/contrast mechanism

Detected secondary electrons

Channel 1 - content

Jurkat cell line E6.1 (artificial color table, cyan)

Channel 1 - biological entity

Surface of Jurkat cells

Channel 2 - content

Raji B cell lymphoma cell line (artificial color table, yellow)

Channel 2 - biological entity

Surface of a Raji B cell

Image acquisition

Instrument attributes

FEI XL30 FEG ESEM

Image acquisition parameters

10 keV, Magnification 6500 x, Scale bar: 2 µm

Submitted via NFDI4BIOIMAGE

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