NFDI4BIOIMAGE Calendar July 2025
Creators
- 1. Molecular Biophysics, B CUBE - Center for Molecular and Cellular Bioengineering, Technical University Dresden, Germany
- 2. General Microbiology, Technical University Dresden, Germany
Contributors
Data curator:
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1.
Heinrich Heine University Düsseldorf
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2.
NFDI4BIOIMAGE
Description
Image from the NFDI4BIOIMAGE Calendar July 2025.
The sample was provided through a collaboration with the group of Thorsten Mascher at TU Dresden. Aim of this project is to explore the cellular autofluorescence patterns in Streptomyces using advanced imaging techniques. Streptomyces coelicolor are multicellular, mycelial bacteria that grow as vegetative hyphae. The use of confocal microscopy in this project was crucial for optically sectioning these filamentous cells, enabling the resolution of their cellular autofluorescence patterns with a high signal-to-noise ratio, which allowed us to visualize the 3D arrangement of the hyphae.
Image Metadata (using REMBI template):
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Study |
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Study type |
Characterization of the intrinsic autofluorescence in filamentous actinobacteria |
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Study Component |
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Imaging method |
Spinning Disk Confocal Microscopy |
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Biosample |
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Biological entity |
Hyphae |
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Organism |
Streptomyces coelicolor M600 |
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Intrinsic variable |
Plasmid free derivative of the wild type strain |
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Experimental variables |
Live-Cell imaging |
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Specimen |
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Preparation method |
S. coelicolor was grown in maltose-yeast extract-malt extract (MYM) medium with tap and deionized water (1:1) and supplemented with 0.2 mL R2 trace element solution per 100 mL. Cultures were inoculated from spore suspension and grown for 18 hours at 28 °C. 2 µl cell suspension was immobilized on 1% agarose pads and covered with a cleaned coverslip (1.5H). |
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Channel 1 - content |
Cellular autofluorescence |
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Channel 1 - biological entity |
S. coelicolor hyphae |
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Image acquisition |
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Instrument attributes |
Imaging was performed using a Nikon Ti-E Spinning Disk microscope with 100x objective and 1.5x tube lens. Fluorescence was excited with a 488 nm laser and emission light was filtered using a dual band filter 433/530 HC. An Andor Ixon Ultra 888 EMCCD camera was used for detection. |
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Image acquisition parameter |
Z-stacks of confocal images with 0.2 µm step size |
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Image data |
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Type |
Maximum intensity projection of individual z-stacks |
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Format & compression |
TIFF |
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Dimension extents |
x: 1024 y: 1024 z: 28 px |
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Size description |
x: 63.12 y: 63.12 z: 5.6 µm |
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Pixel/voxel size description |
x: 86 y: 86 z: 200 nm |
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Submitted via NFDI4BIOIMAGE |
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Files
image_july.png
Files
(35.6 MB)
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