Published August 28, 2025 | Version v1
Poster Open

CARACTERIZAÇÃO GENÔMICA DE Serratia marcescens LB1: POTENCIAIS GENES ASSOCIADOS AO BIOCONTROLE E PROMOÇÃO DO CRESCIMENTO VEGETAL

Creators

  • 1. ROR icon Universidade Estadual Paulista (Unesp)
  • 2. São Paulo State University

Description

O uso intensivo de defensivos agrícolas, com potenciais impactos ambientais e à saúde, tem ampliado a demanda por alternativas sustentáveis, baseadas em bioinsumos. Nesse contexto, destaca-se a necessidade de prospecção e a caracterização de Bactérias Promotoras de Crescimento de Plantas (BPCPs) capazes de estimular o desenvolvimento vegetal e inibir fitopatógenos. Entre elas, o gênero Serratia, reconhecido pela produção de compostos bioativos, destaca-se pelo potencial para aplicações biotecnológicas. Este estudo avalia uma cepa de S. marcescens quanto ao potencial genômico relacionado à sua capacidade de promover crescimento vegetal e inibir fungos fitopatogênicos.

Apresentado no XXXVII Congresso de Iniciação Científica da Unesp – CIC 2025

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References

  • AJITHKUMAR, Bindu et al. Spore-forming Serratia marcescens subsp. sakuensis subsp. nov., isolated from a domestic wastewater treatment tank. International Journal of Systematic and Evolutionary Microbiology, v. 53, n. 1, p. 253-258, 2003.
  • AUCH, Alexander F. et al. Genome BLAST distance phylogenies inferred from whole plastid and whole mitochondrion genome sequences. BMC Bioinformatics, v. 7, n. 1, p. 350, 2006.
  • BOWERS, Robert M. et al. Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea. Nature Biotechnology, v. 35, n. 8, p. 725-731, 2017.
  • CARVER, Tim et al. DNAPlotter: circular and linear interactive genome visualization. Bioinformatics, v. 25, n. 1, p. 119-120, 2009.
  • CHEN, Yu et al. Accurate long-read de novo assembly evaluation with Inspector. Genome Biology, v. 22, n. 1, p. 312, 2021.
  • DIBELLI, S. G. Evidenciação de múltiplos traços de promoção de crescimento vegetal em isolados de comunidades bacterianas associadas a cana-de-açúcar. 2023.
  • FELDGARDEN, Michael et al. Validating the AMRFinder tool and resistance gene database by using antimicrobial resistance genotype-phenotype correlations in a collection of isolates. Antimicrobial Agents and Chemotherapy, v. 63, n. 11, p. e00483-19, 2019.
  • FRIEDRICH, Ines et al. Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog's lettuce (Groenlandia densa). PLoS One, v. 16, n. 11, p. e0259673, 2021.
  • HAFT, Daniel H. et al. RefSeq: an update on prokaryotic genome annotation and curation. Nucleic Acids Research, v. 46, n. D1, p. D851-D860, 2018.
  • HUNT, Martin et al. Circlator: automated circularization of genome assemblies using long sequencing reads. Genome Biology, v. 16, n. 1, p. 294, 2015.
  • KOLMOGOROV, Mikhail; YUAN, Jeffrey; LIN, Yu; PEVZNER, Pavel. Assembly of long error-prone reads using repeat graphs. Nature Biotechnology, 2019. doi:10.1038/s41587-019-0072-8.
  • LI, H.; HOMER, N. A survey of sequence alignment algorithms for next-generation sequencing. Briefings in Bioinformatics, 2010.
  • LI, Wenjun et al. RefSeq: expanding the Prokaryotic Genome Annotation Pipeline reach with protein family model curation. Nucleic Acids Research, v. 49, n. D1, p. D1020-D1028, 2021.
  • MEIER-KOLTHOFF, Jan P.; GÖKER, Markus. TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy. Nature Communications, v. 10, n. 1, p. 2182, 2019.
  • OXFORD NANOPORE TECHNOLOGIES. Dorado. 13 ago. 2025. Disponível em: https://github.com/nanoporetech/dorado .
  • PATZ, S. et al. PLaBAse: A comprehensive web resource for analyzing the plant growth-promoting potential of plant-associated bacteria. 15 dez. 2021. Disponível em: http://biorxiv.org/lookup/doi/10.1101/2021.12.13.472471 . Acesso em: 4 jun. 2024.
  • PELLEGRINETTI, T. A. et al. PGPg_finder: A comprehensive and user-friendly pipeline for identifying plant growth-promoting genes in genomic and metagenomic data. Rhizosphere, v. 30, p. 100905, jun. 2024.
  • SCHMIEDER, Robert; EDWARDS, Robert. Quality control and preprocessing of metagenomic datasets. Bioinformatics, v. 27, n. 6, p. 863-864, 2011.
  • SOENENS, A.; IMPERIAL, J. Biocontrol capabilities of the genus Serratia. Phytochemistry Reviews, v. 19, n. 3, p. 577–587, jun. 2020.
  • TATUSOVA, Tatiana et al. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Research, v. 44, n. 14, p. 6614-6624, 2016.
  • TEHERAN-SIERRA, L. G. et al. Bacterial communities associated with sugarcane under different agricultural management exhibit a diversity of plant growth-promoting traits and evidence of synergistic effect. Microbiological Research, v. 247, p. 126729, 2021.