Genome-Wide Identification, Structural Characterization and Phylogenetic Analysis of Dof (DNA-Binding with one finger) Transcription Factor in Lima Bean (Phaseoulus Lunatus L.)

Dofs are plant-specific transcription factors involved in diverse functions including growth, development, hormonal and Abiotic stress response regulation. Although genome-wide analysis of Dof genes has been performed in many species, but these genes have yet to be analyzed in lima bean. The current study was aimed at exploring and characterizing Dof gene family across the genome of lima bean. Fourty PlDof genes in genome of lima bean were detected, during the present study, and named as PlDof1-PlDof40 on the basis of their location on chromosome in the ascending order. PlDof12 was recorded as the largest gene with 1560 bp CDS, 519 amino acids protein length (PL) and 57.126 kDa protein molecular weight (PMW), while PlDof-23 was found as the smallest one with only 522 CDS, 173 PI and 19.75 kDa PMW, respectively. Nucleus was predicted as a major residence for all 40 PlDof proteins. Isoelectric points and GRAVY were found in the range of 5.35 to 8.91 and -0.269 to -0.746, respectively. The varying number of exons reveal that deletion/addition have been occurred during course of evolution, which resulted variation in motif number. Motif1was conserved in all PlDof genes. Phylogenetic tree reveals that paralogs contributed 33% to the PlDof gene family expansion.  Ka/Ks ratios of paralogs reveal that they were all under purifying selection. Promoter region has environmental, developmental, light and hormone responsive elements that might be contributed to the diverse function of PlDof. This study provide base for functional validity of PlDof genes.