Published May 11, 2025 | Version v1
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HPLC-MS Protocol for Paracetamol Quantification: A Step- by-Step Laboratory Guide

Authors/Creators

  • 1. university of bradford

Contributors

Rights holder:

  • 1. ROR icon University of Bradford

Description

A reproducible HPLC-MS method for paracetamol quantification in tablets was validated using standard addition to address matrix effects. The protocol employs a Kinetex C18 column (water/methanol/acetic acid) with ESI+-MS detection (15-min runtime). Achieved linearity (R² = 0.999), recovery (99% ± 1.2%), and excipient screening (caffeine/aspirin). Ideal for pharmaceutical QC and teaching labs, with open data available for calibration and chromatograms.

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Dates

Issued
2025-05-11

References

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