Published April 26, 2025
| Version v3
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Expanding and improving analyses of nucleotide recoding RNA-seq experiments with the EZbakR suite
Contributors
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Supervisors:
Description
Data necessary to reproduce figures in manuscript titled "Expanding and improving analyses of nucleotide recoding RNA-seq experiments with the EZbakR suite". Includes:
- Compressed arrow dataset used to produce Figure S6 and S7 (subtlseq_data.tar.gz)
- Compressed arrow dataset used to produce Figure 7 (subtlseq_perturbations_data.tar.gz)
- eCLIP DDX3X peak calls from ENCODE used in Figure 7 (ENCFF901BYH_DDX3X_eCLIP.bed)
- Annotations used to process data (Hs_ensembl_lvl1_and_2.gtf and Hs_ensembl.gtf)
- Processed data to produce Figures 3C and S2 (cB_ensembl_totRNAsubtlseq.csv.gz and cB_ensemblLvl1and2_totRNAsubtlseq.csv.gz, respectively).
- Simulated data originally used in bakR publication (Vock and Simon, 2023) used to make Figure 6 of EZbakR suite paper.
- Processed data from the nanodynamo paper (Tarrerro et al. 2024) used to make Figure S9
- Table from Ietswaart et al. 2024 of kinetic parameter estimates and PUND calls from that study (mmc2.xlsx)
Also includes supplemental tables of:
- List of genes producing transcripts predicting to undergo nuclear decay (PUNDs; Supplemental_Table_PUNDs.csv)
- Estimates for mature RNA synthesis, nuclear degradation, nuclear export, and cytoplasmic degradation rate constants from Ietswaart et al., 2024 total-cytoplasmic-nuclear TimeLapse-seq dataset (Supplemental_Table_NucCytoEsts.csv)
- Estimates for premature RNA synthesis, premature RNA processing, and mature RNA degreadation obtained from EZbakR analysis of Ietswaart et al., 2024 total RNA TimeLapse-seq dataset (Supplemental_Table_PtoMests.csv).
Scripts to reproduce figures can be found at: https://github.com/isaacvock/EZbakRsuite_paper_code
Updated to include data necessary to reproduce new figures/panels in revisions.
Files
Supplemental_Table_NucCytoEsts.csv
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Additional details
Funding
- National Institutes of Health
- National Heart, Lung, and Blood Institute Division of Intramural Research
Dates
- Submitted
-
2025-04-26Update for revisions
Software
- Repository URL
- https://github.com/isaacvock/EZbakRsuite_paper_code
- Programming language
- R
- Development Status
- Active