Chromatogram alignments of white lupin genome contigs and PCR products obtained for PR_74, PR_76, PR_77, PR_78, PR_79 and PR_80 markers using DNA isolated from representative white lupin lines

PR_74 marker

 

White lupin pangenome contigs:

LD37_contig_002595 (white lupin line LD37)

P27174-4_contig_000651 (white lupin line P27174)

LalbFTa1_Chr02 white lupin line Amiga)

 

White lupin PCR products:

PR74-24_PR74-F.ab1 (forward): primer PRFTa1_F1c, white lupin line “24”, accession number “95187”, name “BGRC-3911”, single product

PR74-119c_PR74-F.ab1 (forward): primer PRFTa1_F1c, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product

PR74-24_PR74-R.ab1 (reversed): primer PRFT1a_F2aR0, white lupin line “24”, accession number “95187”, name “BGRC-3911”, single product

PR74-119c_PR74-R.ab1 (reversed): primer PRFT1a_F2aR0, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product

PR74-D_PR74-F.ab1 (forward): primer PRFTa1_F1c, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR74-K_PR74-F.ab1 (forward): primer PRFTa1_F1c, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

PR74-D_PR74-R.ab1 (reversed): primer PRFT1a_F2aR0, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR74-K_PR74-R.ab1 (reversed): primer PRFT1a_F2aR0, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

 

PR_76 marker

 

White lupin pangenome contigs:

LD37_contig_002595_: (white lupin line LD37)

P27174-4_contig_: (white lupin line P27174)

LalbFTa1_Chr02_: (white lupin line Amiga)

 

White lupin PCR products:
PR76-19-3_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “19”, accession number “95149”, name “AL-22”,the shortest product

PR76-14-3_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “14”, accession number “95130”, name “Population-84152”, the shortest product

PR76-14-3_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “14”, accession number “95130”, name “Population-84152”, the shortest product

PR76-19-3_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “19”, accession number “95149”, name “AL-22”, the shortest product

PR76-D_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR76-K_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

PR76-K_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

PR76-D_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR76-24_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “24”, accession number “95187”, name “BGRC-3911”, single product

PR76-119c_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product

PR76-24_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “24”, accession number “95187”, name “BGRC-3911”, single product

PR76-119c_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product

PR76-19-2_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “19”, accession number “95149”, name “AL-22”, intermediate product

PR76-19-2_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “19”, accession number “95149”, name “AL-22”, intermediate product

PR76-14-2_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “14”, accession number “95130”, name “Population-84152”, intermediate product

PR76-14-2_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “14”, accession number “95130”, name “Population-84152”, intermediate product

 

PR_77 marker

 

White lupin pangenome contigs:

GR38_contig_001073: (white lupin line GR38)

LalbFTa2_Chr21: (white lupin line Amiga)

 

White lupin PCR products:

PR77-17c_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “LAP017c”, accession number “LAP017c”, name “Gr33”, single product

PR77-17d_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “LAP017d”, accession number “LAP017d”, name “Gr33”, single product

PR77-17d_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “LAP017d”, accession number “LAP017d”, name “Gr33”, single product

PR77-17c_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “LAP017c”, accession number “LAP017c”, name “Gr33”, single product

PR77-64-1_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “64”, accession number “95602”, name “Population-775”, longer product

PR77-64-1_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “64”, accession number “95602”, name “Population-775”, longer product

PR77-26c_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “LAP026c”, accession number “LAP026c”, name “Tr21”, single product

PR77-26c_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “LAP026c”, accession number “LAP026c”, name “Tr21”, single product

PR77-65_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “65”, accession number “95605”, name “Population-743”, single product

PR77-65_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “65”, accession number “95605”, name “Population-743”, single product

PR77-K_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

 

PR77-K_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

PR77-D_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR77-D_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR77-64-2_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “64”, accession number “95602”, name “Population-775”, shorter product

PR77-64-2_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “64”, accession number “95602”, name “Population-775”, shorter product

 

PR_78 marker

 

White lupin pangenome contigs:

GR38_contig_001073: (white lupin line GR38)

LalbFTa2_Chr21: (white lupin line Amiga)

 

White lupin PCR products:

PR78-D_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR78-D_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR78-K_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

PR78-K_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

PR78-64-3_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “64”, accession number “95602”, name “Population-775”, the shortest product

PR78-64-3_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “64”, accession number “95602”, name “Population-775”, the shortest product

PR78-115c-3_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, the shortest product

PR78-115c-3_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, the shortest product

PR78-17c_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “LAP017c”, accession number “LAP017c”, name “Gr33”, single product

PR78-65_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “65”, accession number “95605”, name “Population-743”, single product

PR78-17c_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “LAP017c”, accession number “LAP017c”, name “Gr33”, single product

PR78-65_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “65”, accession number “95605”, name “Population-743”, single product

PR78-64-2_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “64”, accession number “95602”, name “Population-775”, intermediate product

PR78-64-2_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “64”, accession number “95602”, name “Population-775”, intermediate product

PR78-115c-2_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, intermediate product

PR78-115c-2_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, intermediate product

 

PR_79 marker

 

White lupin pangenome contigs:

Clovis_contig_006417: (white lupin line Clovis)

LalbFTa2_Chr21: (white lupin line Amiga)

 

White lupin PCR products:

PR79-D_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR79-K_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

PR79-K_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

PR79-D_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR79-115c-3_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, the shortest product

PR79-48-3_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “48”, accession number “95468”, name “Azurnyj”, the shortest product

PR79-115c-3_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, the shortest product

PR79-119c_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product

PR79-119d_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “LAP119d”, accession number “LAP119c”, name “Aster”, single product

PR79-119c_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product

PR79-119d_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “LAP119d”, accession number “LAP119c”, name “Aster”, single product

PR79-115c-2_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, intermediate product

PR79-115c-2_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, intermediate product

PR79-48-2_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “48”, accession number “95468”, name “Azurnyj”, intermediate product

 

PR_80_marker

 

White lupin pangenome contigs:

LD37_contig_001630: (white lupin line LD37)

P27174-4_contig_001786: (white lupin line P27174)

LalbFTc1_Chr14: (white lupin line Amiga)

GR38_contig_001107: (white lupin line GR38)

White lupin PCR products:

PR80-18d_PR80-F.ab1 (forward): primer PRFTc1_F2e, white lupin line “LAP018d”, accession number “LAP018d”, name “Gr49”, single product

PR80-18d_PR80-R.ab1 (reversed): primer PRFTc1_R2e, white lupin line “LAP018d”, accession number “LAP018d”, name “Gr49”, single product

PR80-22e_PR80-F.ab1 (forward): primer PRFTc1_F2e, white lupin line “LAP022e”, accession number “LAP022e”, name “Tr7”, single product

PR80-22e_PR80-R.ab1 (reversed): primer PRFTc1_R2e, white lupin line “LAP022e”, accession number “LAP022e”, name “Tr7”, single product

PR80-D_PR80-F.ab1 (forward): primer PRFTc1_F2e, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR80-D_PR80-R.ab1 (reversed): primer PRFTc1_R2e, white lupin line “D”, accession number “95519”, name “P27174”, single product

PR80-K_PR80-F.ab1 (forward): primer PRFTc1_F2e, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

PR80-K_PR80-R.ab1 (reversed): primer PRFTc1_R2e, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product