Published March 18, 2025 | Version v1
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Reconstructed images of a 2DSIM multiposition acquisition.

Description

Acquired with an Nikon SIM, in 2D-SIM mode at 488nm of excitation with 30% laser power and 200ms of exposition.  Fluorescence is mStayGold-β2Spectrin, expressed with different maners, either infection via Cas9 or nucleofection with Cas9. Cells are rat hippocampal neurons at different DIVs. The files are uploaded to show and provide bugged files that open as static noise in ImageJ BioFormats 8.1.1. N1 and N2 files are files encountering the issue. N3 and N4 files do not encounter the issue and are uplodaded for comparison purposes. Raw files open as they should, while the issue is always encountered on reconstructed files.

Files

Files (1.4 GB)

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md5:d9dc59fa4cedca1aea1cfffad8cdf78b
94.6 MB Download
md5:5a55479c462e22bae9d5287620e160be
84.2 MB Download
md5:0665c92405ec3f8fd1a9b4b0b4d91b4c
113.5 MB Download
md5:005f1ec4694ec4abeeb02776cf195f55
100.9 MB Download
md5:901392227922d65c501b6b779064f85c
340.0 MB Download
md5:88694a0e82b6b505331113a0df7861a2
302.3 MB Download
md5:1465e5b8fd18eb6bcfec406498adf72a
189.0 MB Download
md5:b12a2eddcb4959984a7899845709d594
168.1 MB Download