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Published March 10, 2025 | Version v0.6.2
Software Open

github.com/iwc-workflows/fastq-to-matrix-10x/scrna-seq-fastq-to-matrix-10x-cellplex

  • 1. UNIGE
  • 2. University of Freiburg
  • 3. Friedrich Miescher Institute for Biomedical Research
  • 4. The Open University
  • 5. Lerner Research Institute , Cleveland Clinic

Description

Comprehensive preprocessing for 10X Genomics CellPlex multiplexed single-cell RNA-seq data. Processes Cell Multiplexing Oligo (CMO) FASTQ files with CITE-seq-Count including required CellPlex-specific translation steps. Simultaneously processes gene expression FASTQ files with STARsolo and DropletUtils for alignment and cell filtering, and formats outputs for seamless import into Seurat/Scanpy (Read10X function).

Files

github.com-iwc-workflows-fastq-to-matrix-10x-scrna-seq-fastq-to-matrix-10x-cellplex_v0.6.2.zip

Additional details