Published December 10, 2024 | Version v1
Dataset Open

Research data supporting "Synaptophysin accelerates synaptic vesicle fusion by expanding the membrane upon neurotransmitter loading"

Creators

  • 1. ROR icon Universitätsmedizin Göttingen
  • 2. ROR icon University of Virginia
  • 3. ROR icon Charité - Universitätsmedizin Berlin
  • 4. University of Virginia School of Medicine
  • 5. Max-Planck-Institute for Multidisciplinary Sciences
  • 6. ROR icon Leibniz-Forschungsinstitut für Molekulare Pharmakologie
  • 7. University of Virginia, Charlottesville
  • 8. ROR icon Max Planck Institute for Multidisciplinary Sciences

Description

Research data supporting Preobraschenski et al. "Synaptophysin accelerates synaptic vesicle fusion by expanding the membrane upon neurotransmitter loading"

Synaptic transmission is mediated by the exocytotic release of neurotransmitters stored in synaptic vesicles (SVs). SVs filled with neurotransmitters preferentially undergo exocytosis but it is unclear how this is achieved. Here we show that during transmitter loading SVs substantially increase in size, which is reversible and requires synaptophysin, an abundant membrane protein with unclear function. SVs are larger when synaptophysin is knocked out, and conversely, liposomes are smaller when reconstituted with synaptophysin. Moreover, transmitter loading of SVs accelerates fusion in vitro which is abolished when synaptophysin is lacking despite near normal transmitter uptake. We conclude that synaptophysin functions as a curvature-promoting entity in the SV membrane, allowing for major lateral expansion of the SV membrane during neurotransmitter filling, thus increasing their propensity for exocytosis.

Files

Fig. 4D.pdf

Files (1.9 MB)

Name Size Download all
md5:c55d794839fae2a003c987a1f576c6e4
11.8 kB Download
md5:b6969d234bc1cd9e8977db080acf9e5c
28.5 kB Download
md5:3fdfa137a0c12cbd041a2b4e44398bf6
16.7 kB Download
md5:d14c4885e0de9f0665f5d7668f2bca82
1.1 MB Download
md5:66547c2bc91d0ed5eafed8d47c566029
9.3 kB Download
md5:3345398f9a028672f8ce0e4b9be7d421
9.7 kB Download
md5:a6833e4bec0c4a8f87d444cb414ec5a1
37.6 kB Download
md5:2f6f9def9a7aea9f15a64770674e7431
551.0 kB Preview Download
md5:9a36145ba20bd43dbc39ba1f79c8e8a5
10.3 kB Download
md5:3ecf2b894741dfe7ff29e9cd78656432
9.3 kB Download
md5:23cf32708cd40edd17877f99cac971d7
8.9 kB Download
md5:8be3fa94375046d62e92bdd64d495bc9
10.2 kB Download
md5:81fba615a347a242a590a36961c6ac8c
27.3 kB Download
md5:e5eae1fc2840637f7b90314b19693487
22.6 kB Download
md5:5905d87b841656746649e8ffd9de8be4
10.7 kB Download
md5:8058314c52a7d9acbd72ade2cb5faca8
14.5 kB Download
md5:90c2889d6febefdc1ce7fbc15051c1e9
11.0 kB Download
md5:997e4887481e5bae7786dd5e386b225a
10.0 kB Download

Additional details

Software

Repository URL
https://github.com/VolkerKirchheim/Cryo_Bilayer_1.git