REVIEW ARTICLE ON UV-VISIBLE SPECTROSCOPY IN PHARMACEUTICAL ANALYSIS METHODOLOGIES AND APPLICATIONS

UV-Visible spectroscopy refers to the analytical examination of various solvents and compounds. The analysis involves measuring the absorption of monochromatic light by colourless and coloured molecules in the region of the spectrum 200–400 nm and 400 to 800nm respectively, the principle of the technique is based on the electronic transitions of molecules, where photons in the UV and visible regions promote electrons from lower energy orbitals to higher ones. The methodology involves passing a beam of light through a sample and measuring the intensity of light before and after it interacts with the sample. The resulting absorbance spectra can be utilized to identify compounds, quantify concentrations using Beer-Lambert law, and assess chemical reactions. Factors such as the solvent, pH, and temperature can affect the absorption characteristics, making careful experimental design crucial. Recent advancements in UV-visible spectroscopy include the development of more sensitive detectors, integration with other analytical techniques, and the ability to conduct real-time measurements, broadening its applicability in research and industry.

 This technique used to determine the "identity, strength, quality, and purity" of such compounds UV-visible spectroscopy is a widely employed analytical technique used to measure the absorbance or transmittance of ultraviolet and visible light by the sample. This technique is essential in various fields, including chemistry, biochemistry, and environmental science, as it provides valuable insights into the electronic structure of compounds, concentration analysis, and chemical kinetics.

This abstract encapsulates the significance and versatility of UV-visible spectroscopy as an essential tool in scientific analysis ⁽¹⁾

Keywords: UV-Vis Spectroscopy, Optimization Techniques, Electrochemical Methods.