Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection
Creators
- 1. Regulatory Research Division, Biologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch, Health Canada
- 2. Univ. Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille
- 3. Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa
- 4. School of Pharmaceutical Sciences, Faculty of Medicine, University of Ottawa
- 5. Laboratory of Immunoinflammation, Institute of Biology, University of Campinas (UNICAMP)
Description
The gut microbiome's pivotal role in health and disease is well-established. SARS-CoV-2 infection often causes gastrointestinal symptoms and is associated with changes of the microbiome in both human and animal studies. While hamsters serve as important animal models for coronavirus research, there exists a notable void in functional characterization of their microbiomes with metaproteomics. In this study, we present a workflow for analyzing the hamster gut microbiome, including a metagenomics-derived hamster gut microbial protein database and a data-independent acquisition metaproteomics method. Using this workflow, we identified 32419 protein groups from the fecal microbiomes of young and old hamsters infected with SARS-CoV-2 . We showed age-specific changes in the expressions of microbiome functions and host proteins associated with microbiomes, providing further functional insight into the dysbiosis and aberrant cross-talks between the microbiome and host in SARS-CoV-2 infection. Altogether this study established and demonstrated the capability of metaproteomics for the study of hamster microbiomes.