Production of Bioethanol from Watermelon and Banana peels

Bioethanol, an ethanol liquid that is known to be a clean fuel for combustion engines, is a readily available substitute since it can be derived from plant-based materials. is the most commonly used alternative transportation fuel to petrol. It can help to mitigate climate change and reduce environmental pollution. In this study, pretreated fruits peels were enzymatically hydrolysed using Aspergillus niger from soil. Saccharomyces cerevisiae from palm wine was used for fermentation. The hydrolysate was used to produce bioethanol which was determined by HPLC analysis. HPLC analysis was carried out to determine the presence of bioethanol produced. Peak area of the distillated bioethanol produced was compared with that of a standard ethanol. Bioethanol produced from the hydrolysate of fruits peels using Saccharomyces cerevisiae showed highest yield of (1.36%) from biologically pretreated fruit peels and hot water pretreatment produced a low bioethanol concentration of (0.16%). Bioethanol was produced in the fermentation broth with High Performance Liquid Chromatography (HPLC) peak of 2.548min for watermelon and 2.234min for banana. highest reducing sugar yield was achieved with sample of Bacillus subtilis pretreatment.