Published September 11, 2024 | Version v1
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Scripts used in microbiome analysis in Schizaphis graminum

  • 1. USDA-ARS

Description

These scripts were used to download reference and representative microbial genomes from NCBI, attribute RNAseq counts to a closest hit among multiple sam files from bwa mem runs, and aggregate RNAseq counts by hit taxon at various levels in a taxonomic hierarchy.  They were used in a general strategy to count organisms based on unamplified transcripts mapped in parallel to tens of thousands of whole genomes.  A manuscript has been submitted to Insect Molecular Biology to report the results of this study.

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Additional details

Related works

Cites
Journal article: 10.1371/journal.pone.0294013 (DOI)

Dates

Submitted
2024-09-11
research article