Evaluation of Wound Healing Potency of Dipteracanthus patulus (Jacq) Leaf Extracts Using Chick Embryo Wound Model

INTRODUCTION:

Wound healing is a critical process in tissue repair, and traditional medicinal plants offer promising therapeutic potential. Dipteracanthus patulus (Jacq) is a plant with known medicinal properties, but its wound healing effects is not well studied. This study evaluates the wound healing potency of its leaf extracts using the chick embryo wound model.

MATERIALS AND METHOD:

D.patulus leaf collected was dried at room temperature under shade 15 days and coarsely powdered. The powdered materials were extracted with ethanol and chloroform. The last traces of the solvent were removed and concentrated to dryness under vacuum using a rotary evaporator. The dried extract was weighed and then kept at -4ºC until ready for use. The yield of the extract was 66.42 % (w/w) and 33.68 % (w/w). In each experiment, the extract was diluted with water to desired concentration.

 RESULT:

In Chorioallantoic membrane Assay model, the result was tabulated by counting the number of blood vessels in various treatments. Ethanolic and chloroform extract, positive control promoted an increase in number of blood vessels compared to negative control saline. Treated 500μg/ml. Ethanolic and chloroform extract group and 50μg/ml Diclofenac sodium positive control treated group was observed formation of new blood vessels.

CONCLUSION:

Dipteracanthus patulus both extract stimulated angiogenesis as proved by CAM model showed a faster wound contraction. By comparing both the extracts, ethanolic extract show better activity than the chloroform extract. Dipteracanthus patulus leaf extracts was found to possess angiogenesis.