The Study of Megakaryocytes in Bone Marrow Aspiration Smears in Patients with Thrombocytopenia
Creators
- 1. Assistant Professor, Department of Pathology, MKCG Medical College & Hospital, Brahmapur
- 2. Associate Professor, Department of Pathology, MKCG Medical College & Hospital, Brahmapur
Description
Background: Thrombocytopenia is a common hematology condition for which bone marrow aspiration is indicated. Thrombocytopenia is encountered in various hematological disorders including MDS as well as non-MDS. Dysmegakaryopoiesis is characterized by various megakaryocytic alterations in bone marrow aspirates and include both dysplastic and non-dysplastic features. Methods: A prospective study was conducted in cases of non-MDS related thrombocytopenia and the bone marrow aspirates were studied morphologically. The cases of thrombocytopenia (platelet count <150,000 per microliter or <150 x 109/L or <1.5 K/cumm) was done by automated platelet count and were further confirmed manually in peripheral smear. Bone marrow aspiration was done in confirmed thrombocytopenia cares and the morphology of megakaryocytes was studied. Results: A total of 98 cases of thrombocytopenia were included in the present study predominated by megaloblastic anemia. 43 cases, followed by acute leukemia, 30 cases (AML-16, ALC-14), Aplastic anemia (12 cases), ITP (11 cases) and multiple myeloma (2 cases). Dysmegakaryopoesis in the form of nuclear separation, bare nuclei, micromegakaryocytes, emperipolesis, and vacuolization were observed in the present study. The evaluation of megakaryocyte alternation in morphology in cases of thrombocytopenia can provide more knowledge to the pathogenesis of various hematopoietic disorders.
Abstract (English)
Background: Thrombocytopenia is a common hematology condition for which bone marrow aspiration is indicated. Thrombocytopenia is encountered in various hematological disorders including MDS as well as non-MDS. Dysmegakaryopoiesis is characterized by various megakaryocytic alterations in bone marrow aspirates and include both dysplastic and non-dysplastic features. Methods: A prospective study was conducted in cases of non-MDS related thrombocytopenia and the bone marrow aspirates were studied morphologically. The cases of thrombocytopenia (platelet count <150,000 per microliter or <150 x 109/L or <1.5 K/cumm) was done by automated platelet count and were further confirmed manually in peripheral smear. Bone marrow aspiration was done in confirmed thrombocytopenia cares and the morphology of megakaryocytes was studied. Results: A total of 98 cases of thrombocytopenia were included in the present study predominated by megaloblastic anemia. 43 cases, followed by acute leukemia, 30 cases (AML-16, ALC-14), Aplastic anemia (12 cases), ITP (11 cases) and multiple myeloma (2 cases). Dysmegakaryopoesis in the form of nuclear separation, bare nuclei, micromegakaryocytes, emperipolesis, and vacuolization were observed in the present study. The evaluation of megakaryocyte alternation in morphology in cases of thrombocytopenia can provide more knowledge to the pathogenesis of various hematopoietic disorders.
Files
IJPCR,Vol14,Issue7,Article104.pdf
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Additional details
Dates
- Accepted
-
2022-06-05
Software
- Repository URL
- https://impactfactor.org/PDF/IJPCR/14/IJPCR,Vol14,Issue7,Article104.pdf
- Development Status
- Active
References
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