Laboratory of Translational Oncology - Gdańsk Medical University
Published August 26, 2024 | Version v1
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CTC identification and characterization in prostate cancer using K/V protocol and imaging flow cytometry - part 4

Authors/Creators

  • 1. ROR icon Gdańsk Medical University

Description

This project aimed to identify and characterize circulating tumor cells (CTCs) in peripheral blood of 33 prostate cancer patients. Fraction of peripheral blood mononuclear cells putatively containing CTCs was isolated using density gradient centrifugation. Pan-keratin/vimentin/CD45/CD31/DAPI immunofluorescent staining was performer and DAPI+ or DAPI+CD45/CD31- objects were collected using Amnis® ImageStream®XMk II, Cytek® Biosciences, Inc (lasers: 405 nm, 488 nm, 642 nm, magnification 40x, slow speed flow).

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Additional details

Funding

National Science Centre
Molecular and clinical characterization of keratin-based phenotypes in prostate cancer #2017/26/D/NZ5/01088
National Science Centre
Identification of potential markers aiming early diagnostics of progression and tailoring metastasis-oriented therapies in prostate cancer (EDMOT) #2020/39/B/NZ5/01258

References

  • doi:10.3390/cancers14174178
  • doi:10.3390/cancers15164169
  • doi: 10.1002/cyto.a.24906