This study investigates the variations in body size, bioacoustic traits, and cuticular hydrocarbon (CHC) profiles in different populations of the bush cricket species Isophya autumnalis Karabağ, 1962. Within-population body size variations, particularly those associated with distinct habitat differences and climate shifts within their local distribution ranges, suggest that ecological factors affect morphological characteristics. Sexual size dimorphism (SSD) in I. autumnalis may affect reproductive behavior and strategies, potentially influenced by the bioacoustic environment. While male calling songs exhibit temporal variations across populations, suggesting differences among allopatric populations, CHC profiles, known to undergo selection under various climatic conditions, also vary noticeably across local populations. These findings highlight the importance of understanding within-species variations for the conservation of Isophya and similar taxa in the face of habitat threats. Overall, this study contributes to a comprehensive understanding of how morphology and bioacoustic behavioral traits are shaped over short distances in allopatric populations of species with limited mobility, such as I. autumnalis, providing insights into adaptation processes and highlighting the urgency of conservation efforts for endemic species in Anatolia.