COMPARATIVE STUDIES ON KINETIC PARAMETERS OF DETOXIFICATION ENZYMES IN THE HIND GUT OF Zonocerus variegatus Linnaeus, 1758
- 1. Department of Biochemistry, Faculty of Science, University of Ilesa, Ilesa, Nigeria.
- 2. Department of Biochemistry and Molecular Biology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria
- 3. Department of Medical Biochemistry, Faculty of Basic Medical Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
Description
AIMS: The grasshopper (Zonocerus variegatus) is a polyphagous insect that feeds on wild, cultivated and uncultivated crops, causing extensive damage during their feeding process. Understanding the presence and expression of various detoxification enzymes in the gut of Z. variegatus (Z.v) may help to understand how they cope with allelochemicals encountered in their feeding process. METHODOLOGY: The grasshoppers were collected and dissected to obtain the hindgut. The hindgut was homogenized in Tris-HCl buffer (pH 9.6) and centrifuged at 10,000 g for 1 h. The supernatant was collected and stored as crude enzyme. Assay for the presence and quantification of the following enzymes: glutathione-S-transferase (GST), peroxidase, lysozyme, catalase, and superoxide dismutase (SOD) were carried out. Specific activities and kinetic parameters of the enzymes were also determined.
RESULTS: GST expression was the lowest amongst the antioxidant enzymes assayed, with a specific activity of 2.32 ± 0.6 Units/mg protein, whereas catalase was most expressed, with a specific activity of 3827.37 ± 201.11 Units/mg protein. The order of expression from largest to lowest was catalase>SOD>lysozyme>peroxidise>GST. CONCLUSION: This study concludes that Zonocerus variegates contains varying amounts of detoxification enzymes, which helps the insect to eliminate both exogenous and endogenous toxins
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