Research data for the article "Membrane-Active Antibiotics Affect Domains in Bacterial Membranes as the First Step of Their Activity" by Adéla Melcrová, Christiaan Klein, and Wouter Roos, Nano Letters 2024.

Research data for the publication Membrane-Active Antibiotics Affect Domains in Bacterial Membranes as the First Step of Their Activity, Nano Letters, 2024. https://doi.org/10.1021/acs.nanolett.4c01873

The data set contains experimental data on the study of antimicrobial N-alkylamide 3d and its interaction with the membrane extracted from the bacteria Staphylococcus aureus. Experimental details can be found in the Methods section of the Supporting Information of the mentioned article. 

The data set contains underlying data for each Figure in the publication including the figures in the Supporting Information. 

• Figure 1, S2, S3, S6, S8 - Atomic Force Microscopy images in the raw format as collected on JPK Nano Wizard Ultra Speed AFM.
• Figure 2, 3, S1 - High-Speed Atomic Force Microscopy images in the raw format as collected on high-speed AFM RIBM machine. The data could be opened in Kodec software. The full trajectories recorded on the high-speed AFM machine are presented here. Only snapshots were used in the publication Figures.
• Figure S7, S9 - Snapshots from the High-Speed AFM measurements showing the cross-section lines (.bmp) together with the cross-section profiles (.csv) that were used to measure the height of supramolecular structures on top of the bacterial membrane.
• Data underlying Figure S5 and Supporting Video 1 overlap with the data presented in Figure 2a.