Published October 31, 2011 | Version v1
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Interlaboratory comparison and validation of detection methods for phytoplasmas of phytosanitary concern in European orchards (FRUITPHYTOINTERLAB)

  • 1. Ministry of Agriculture, Livestock, Fisheries and Food (DAMM), Barcelona, Spain
  • 2. Austrian Agency for Health and Food Safety (AGES), Vienna, Austria
  • 3. Research Institute for Agriculture, Fisheries and Food (ILVO), Merelbeke, Belgium
  • 4. Central Laboratory for Plant Quarantine (CLPQ), Sofia, Bulgaria
  • 5. Agroscope, Nyon, Switzerland
  • 6. State Phytosanitary Administration, Division of Diagnostics (SRS), Prague, Czech Republic
  • 7. Julius Kuehn Institute (JKI), Dossenheim, Germany
  • 8. Aarhus University (AU), Slagelse, Denmark
  • 9. Institute for Food and Agricultural Research and Technology (IRTA), Barcelona, Spain
  • 10. Politechnic University of Valencia (UPV), Valencia, Spain
  • 11. Agricultural Research Council (CRA), Rome, Italy
  • 12. Minoprio Foundation, Vertemate con Minoprio, Italy
  • 13. University of BOlogna (UNIBO), Bologna, Italy
  • 14. Bioforsk, As, Norway
  • 15. National Plant Protection Organization (NVWA), Wageningen, The Netherlands
  • 16. Main Inspectorate of Plant Health and Seed Inspection (PIORIN), Torun, Poland
  • 17. National Institute of Biological Resources (INRB), Lisbon, Portugal
  • 18. Central Control and Testing Institute of Agriculture (UKSUP), Bratislava, Slovakia
  • 19. National Institute of Biology (NIB), Ljubljana, Slovenia
  • 20. The Food and Environment Research Agency (FERA), Sand Hutton, United Kingdom
  • 21. General Directorate of Agricultural Research-Plant Protection Research Institute (GDAR), Izmir, Turkey


Phytoplasmas are specialised plant pathogenic bacteria, colonizing the plant phloem tissue. They lack a cell wall, are non-culturable and are transmitted by insect vectors and by vegetatively propagated plant material. These plant pathogens are increasingly spreading and causing economical losses especially on fruit tree production. Molecular detection methods are already available; however sampling, extraction and detection require harmonisation and validation throughout European laboratories. This process would improve the reliability of results enabling the development of improved management strategies to prevent the spread of these diseases. The project's main objective was to carry out inter-laboratory ring-testing to validate test methods, on 16S and 23S ribosomal gene, for the detection of phytoplasmas (’Candidatus Phytoplasma mali’,’Ca. P. pyri’, ’Ca. P. prunorum’) in symptomatic and asymptomatic plants from infected orchards, including nested-PCR and real time PCR.


Scientific report of the Euphresco funded project 'Interlaboratory comparison and validation of detection methods for phytoplasmas of phytosanitary concern in European orchards' (FRUITPHYTOINTERLAB)



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