Published August 5, 2024 | Version v1
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Supplement to Witting et al. 2024

  • 1. ROR icon Forschungszentrum Jülich

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  • 1. ROR icon Forschungszentrum Jülich

Description

Supplement to Witting et al. 2024

Image sequences are sorted by figure.

Figure 3:

Synechococcus elongatus UTEX2973 was cultivated at 37 °C in BG11 medium under homogeneous growth light illumination.

Data was aquired from distinct experiments under homogeneous growth light illumination.

Image data is sorted by applied light-intensity. Images were taken every 1 h.

Example (.avi) videos for the distinct colonies displayed in subfigures C-E are also uploaded.

Figure 4: 

Cultivation of three cyanobacteria model strains under gradient growth light illumination.

Image data is sorted by cyanobacteria strain.

All strains were cultivated at 37 °C in BG11 medium. Images were taken every 2 h.

Figure 5:

Synechococcus elongatus UTEX2973 was cultivated at 37 °C in BG11 medium. Images were taken every 2 h.

B: Cultivation of S. elongatus UTEX2973 under gradient growth light illumination with and Day/Night (12 h day, 12 h night) Rythm appied

C: Cultivation of S. elongatus UTEX2973 under gradient growth light illumination with decreasing oxygen concentrations

Applied CO2 concentrations:

Time [h] Frame CO2 concentration [ppm]
0 - 24 1 - 13 100
24 - 48 13 - 25 50
48 - 72 25 - 37 15
72 - 96 37 - 49 0

Further files

CAD Files

  • The replication master for molding the gas layer
  • The design of the connector used to mount the Li190R Intensity sensor into the Nikon Ti-E microscope

Files

CAD_Files.zip

Files (23.6 GB)

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Additional details

Funding

Deutsche Forschungsgemeinschaft
SFB1535 458090666
Helmholtz Association of German Research Centres
EMSIG EMSIG ZT-I-PF-04-044