Published August 1, 2024 | Version v1
Dataset Open

Mechanism for Vipp1 spiral formation, ring biogenesis and membrane repair

Creators

  • 1. Biofisika Institute
  • 2. ROR icon University of the Basque Country
  • 3. ROR icon Imperial College London
  • 4. ROR icon University of Geneva
  • 5. ROR icon Ikerbasque

Description

Here, we collect an image dataset of Vipp1 polymerization dynamics on supported lipid bilayers using atomic force microscopy (AFM). A JPK NanoWizard Ultraspeed AFM (Bruker and JPK BioAFM) equipped with USC-F0.3-k0.3-10 cantilevers with spring constant of 0.3N nm−1, resonance frequency of about 300 kHz (Nanoworld), was employed for image acquisition. The AFM was operated in tapping mode with a cantilever oscillation frequency near to 150kHz. Both topographic and phase images were analysed using JPKSPM Data Processing, ImageJ, and WSxM software.

Files

Vipp1_AFM.zip

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