Data and code for: Recombinant venom proteins in insect seminal fluid reduces female lifespan

Published August 1, 2024 | Version v3

Dataset Open

Publication: https://doi.org/10.1101/2024.01.14.575309

This repository contains all the data as well as the scripts used for the analysis and visualisation.

File	Description
TMT.ipynb	The Jupyter notebook containing the Python code for statistical analysis and figure generation. Scripts are sectioned in the order that results are presented in the study, with sub-headings and figure numbers where appropriate.
actingal4.csv	Offspring phenotype data from the UAS:venom x Act5C-GAL4 assay (Results: Testing functional expression of recombinant venoms).
femalelifespan_1-1.csv	Female lifespan data from the 1:1 male:female mating assay (Results: TMT males reduce the lifespan of mated females). Time = days since initial male exposure until death. Status: 1 = death observed; 0 = censored.
femalelifespan_3-1.csv	Female lifespan data from the 3:1 male:female mating assay (Results: TMT males reduce the lifespan of mated females). Time = days since initial male exposure until death. Status: 1 = death observed; 0 = censored.
singlemating.csv	Data from the single-pair courtship assay. 1 = successful courtship observed; 0 = successful courtship not observed.
competitivemating.csv	Offspring genotype data from the competitive mating assay. Female = group of offspring from a given mother. white = positive result from gDNA PCR using the 'white' primers (supplementary table S2). TMT = positive result from gDNA PCR using the 'UAS' primers (supplementary table S2).
malelifespan.csv	TMT male lifespan data from the male longevity assay. Time = days since eclosion. Status: 1 = death observed; 0 = censored.

TMT-GAMA.db contains data from the GAMA model (doi:10.5281/zenodo.11439089), which is accessed by the Jupyter notebook.

Table	Description
TMT_step_output	Each simulation will write key values to this table each cycle once transgenic males have begun to be released.
TMT_female_output	Each female will write key values to this table upon their death once transgeni males have begun to be released.
TMT_PR50	Each simulation will write to this table when/if the female population reaches 50% of its initial size.
TMT_PR95	Each simulation will write to this table when/if the female population reaches 95% of its initial size.

Files

Name	Size	Download all
actingal4.csv md5:ede4567e71d72040b7e271627b609797	434 Bytes	Preview Download
competitivemating.csv md5:4b5ac67789123c13389c562d8ebbfcf1	361 Bytes	Preview Download
femalelifespan_1-1.csv md5:f1ef2e70ee83ff7ca9906c213dacf94d	5.3 kB	Preview Download
femalelifespan_3-1.csv md5:e3b484d965e0015d82c7185c3550a851	2.5 kB	Preview Download
malelifespan.csv md5:d135c92287bf9c02a2bc3fb6cb5f8947	3.3 kB	Preview Download
singlemating.csv md5:d3bbdd1b142b482d435bcc6a48fe0798	2.9 kB	Preview Download
TMT-GAMA.db md5:35e17dfc9fd2da116a6ec0da90840c49	939.8 MB	Download
tmt.ipynb md5:a0a0381b5b289443a017fab9dd0a1509	734.2 kB	Preview Download